Buckwheat : Methods and Protocols / edited by Alexander Betekhtin, Artur Pinski.

Format:

Book

Contributor:

Betekhtin, Alexander., Editor.

Pinski, Artur., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2791

Language:

English

Subjects (All):

Botany.

Agriculture.

Plant Science.

Local Subjects:

Plant Science.

Agriculture.

Physical Description:

1 online resource (X, 140 p. 39 illus., 33 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This detailed volume describes the most recent methodology for buckwheat research. Buckwheat serves as a promising functional food source containing various phenolic compounds, such as rutin, quercetin, and C-glycosylflavones, which have a positive therapeutic or dietary effect for promoting human health. This book explores techniques for studying this pseudocereal, namely the two most widely cultivated species, F. esculentum (common buckwheat) and F. tataricum (Tartary buckwheat). Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensible, Buckwheat: Methods and Protocols is an ideal guide for plant scientists working in this field.

Contents:

Callus Induction Followed by Regeneration and Hairy Root Induction in Common Buckwheat

Immunostaining for Epigenetic Modifications in Fagopyrum Calli

Quantitative Analysis of Epigenetic Modifications in Fagopyrum Nuclei with Confocal Microscope, ImageJ, and R Studio

Phenolic Determination in Proembryogenic Cell Complexes of Buckwheat Morphogenic Cell Culture with Osmium Tetroxide, Toluidine Blue O Dye, and Iron Chloride

Protoplast Isolation, Culture, and Regeneration in Common and Tartary Buckwheat

Immunohistochemical Detection of the Wall Components on the Example of Shoot Apical Meristem of Fagopyrum esculentum and Fagopyrum tataricum

Immunodetection of Cell Wall Components in Studies on Cell Wall Rebuilding in Fagopyrum esculentum and Fagopyrum tataricum

Visualization of Fagopyrum esculentum and Fagopyrum tataricum Chromosomes and Micronuclei

Embryo Rescue Technique in Buckwheat Fagopyrum esculentum Moench.-Comprehensive Embryological Analyses of Common Buckwheat (Fagopyrym esculentum Moench)

The Phenol-Based Protein Extraction Method for Plant Proteomic Studies

Two-Dimensional Gel Electrophoresis in Studies of Flower and Leaf Proteome of Common Buckwheat

Measurement of the Kinetics of Chlorophyll a Fluorescence by an LED-Light Source Fluorimeter, Handy PEA

Measurements of Leaf Gas-Exchange Parameters Using Portable CIRAS‐3 Infrared Gas Analyzer, with a Parkinson Leaf Chamber (PLC6)

Measurement of the Light Intensity and Spectrum Influence on Plant Growth and Secondary Metabolites of Common Buckwheat.

ISBN:

9781071637944

1071637940