Genetically Incorporated Non-Canonical Amino Acids : Methods and Protocols / edited by Yu-Hsuan Tsai, Simon J. Elsässer.

Format:

Book

Contributor:

Tsai, Yu-Hsuan., Editor.

Elsässer, Simon J., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2676

Language:

English

Subjects (All):

Proteins.

Biochemistry.

Local Subjects:

Proteins.

Biochemistry.

Physical Description:

1 online resource (XI, 286 p. 1 illus.)

Edition:

1st ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.

Contents:

Focused Engineering of Pyrrolysyl-tRNA Synthetase-Based Orthogonal Translation Systems for the Incorporation of Various Non-Canonical Amino Acids

Engineering Homogeneous Photoactive Antibodies Fragments

Repurposing Photosensitizer Proteins through Genetic Code Expansion to Facilitate Photo-Biocatalysis

Genetic Encoding of a Fluorescent Non-Canonical Amino Acid as a FRET Donor for Analysis of Deubiquitinase Activities

Creating Selenocysteine-Specific Reporters Using Inteins

Protein Expression with Biosynthesized Non-Canonical Amino Acids

Reprogramming Initiator and Nonsense Codons to Simultaneously Install Three Distinct Non-Canonical Amino Acids into Proteins in E. coli

Encoding Non-Canonical Amino Acids into Phage Displayed Proteins

Genetically Encoded Non-Canonical Amino Acids in Proteins to Investigate Lysine Benzoylation

Semi-Synthesis of Glutamine-Methylated Proteins Enabled by Genetic Code Expansion

Genetic Code Expansion in Mammalian Cells

Generation of Amber Suppression Cell Lines Using CRISPR-Cas9

Genetic Code Expansion in Mammalian Cells through Quadruplet Codon Decoding

Genetically Encoded 1,2-Aminothiol for Site-Specific Modification of a Cellular Membrane Protein via TAMM Condensation

Conformational GPCR BRET Sensors Based on Bioorthogonal Labeling of Non-Canonical Amino Acids

Selective Inhibition of Kinase Activity in Mammalian Cells by Bioorthogonal Ligand Tethering

Site-Specific Incorporation of Sulfotyrosine into Proteins in Mammalian Cells

Small Molecule Phosphine Activation of Protein Function in Zebrafish Embryos with an Expanded Genetic Code

Non-Canonical Amino Acid Incorporation in Mice.

ISBN:

9781071632512

1071632515