Immune Homeostasis : Methods and Protocols / edited by Vijay Kumar.

Format:

Book

Contributor:

Kumar, Vijay., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2782

Language:

English

Subjects (All):

Immunology.

Natural immunity.

Immunospecificity.

Innate Immunity.

Adaptive Immunity.

Local Subjects:

Immunology.

Innate Immunity.

Adaptive Immunity.

Physical Description:

1 online resource (X, 229 p. 333 illus., 326 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This volume discusses different aspects of immune homeostasis regulation and its dysregulation during various diseases, including infections, cancers, neurodegenerative diseases, autoinflammation, autoimmune diseases, and immunodeficiency, along with immunological, genetic, cell biology, and molecular biology techniques designed to understand immune homeostasis at different stages. The chapters in this book cover topics such as T cell subsets; monocyte-myofibroblast immunomodulation in a 3D co-culture system; human peripheral blood mononuclear cells in patients with neurodegenerative diseases; and CRISPR-Cas9 induced gene-editing in primary human monocytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immune Homeostasis: Methods and Protocols is a valuable resource for both novice and experts researchers interested in learning more about this important and developing field. .

Contents:

Immune Homeostasis: A Novel Example of Teamwork

Immune Homeostasis Maintenance through Advanced Immune Therapeutics to Target Atherosclerosis

T Cell Subsets and Immune Homeostasis

Post-Transcriptional Events Orchestrate Immune Homeostasis of CD8+T Cells

A Robust In Vitro Culture Model and Generation of Memory Monocytes

A Robust In Vitro Co-Culture Model for Studying the Intercellular Communication of Neutrophils

Studying Monocyte-Myofibroblast Immunomodulation in a 3D Co-Culture System

Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients

Analysis of Human Peripheral Blood Mononuclear Cells in Patients with Neurodegenerative Diseases

Modulation and Determination of the Status of Inflammasomes in Leishmania-Infected Macrophages

Fluid-Dynamic Culture of Tumor and Immune Cells for More Predictive Infiltration Studies and Immunotherapy Drug Screening

Isolation and Single-Cell Transcriptomic Analysis of Murine Regulatory B Cells

Isolation and Culture of Mouse Primary Microglia and Oligodendrocyte Precursor Cells

Phenotypic and Functional Characterization of Memory CD4+ and CD8+ T Cells after Antigenic Stimulation

CRISPR-Cas9 Induced Gene-Editing in Primary Human Monocytes

Employing CRISPR-Cas9 to Enhance T Cell Effector Function

Assessing Antigen-Specific T Cell Responses through IFN- Enzyme-Linked Immune Absorbent Spot (ELISpot). .

ISBN:

9781071637548

1071637541