Measles and Related Morbilliviruses : Methods and Protocols / edited by Dzwokai Z. Ma, Christian K. Pfaller.

Format:

Book

Contributor:

Ma, Dzwokai Z., Editor.

Pfaller, Christian K., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2808

Language:

English

Subjects (All):

Virology.

Immunology.

Local Subjects:

Virology.

Immunology.

Physical Description:

1 online resource (XIV, 266 p. 3 illus.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This volume covers the latest protocols used to study the molecular biology of morbilliviruses, including cell entry, replication, and virus-host interactions. The chapters in this book also cover methods for studying morbillivirus infection through the use of novel tissue culture and in vivo models; analyzing the biological implications of infection by using primary tissues isolated from infected animals; and exploring diagnostic tools to evaluate humoral immunity against human-pathogenic measles virus (MeV). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Measles and Related Morbilliviruses: Methods and Protocols is a valuable resource for researchers studying MeV, and will be useful to researchers studying other members of Paramyxoviridae and Mononegavirales. .

Contents:

A Quantitative Fusion Assay to Study Measles Virus Entry

A Semi-Quantitative Protein-Fragment Complementation Assay to Study Protein-Protein Interactions of the Polymerase Complex In Cellula

Design and Execution of In Vitro Polymerase Assays for Measles Virus and Related Mononegaviruses

Optical Control of Mononegavirus Gene Expression and Replication

Generation of Defective Interfering Particles of Morbilliviruses Using Reverse Genetics

Molecular Analysis of Copy Back Defective Interfering RNAs of Morbilliviruses

Applying Reverse Genetics to Study Measles Virus Interactions with the Host

Identification of Host Factors of Paramyxoviruses by siRNA Genome-Wide Screens

Dual RNA-seq Analysis of Patients' Cells and Viral Genome After Measles Infection

Investigating Liquid-Liquid Phase Separation in Virus-Generated Inclusion Bodies Using Fluorescence Recovery After Photobleaching of Fluorescently Labeled Host Proteins

Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection

In Vitro Modeling of Feline Morbillivirus Infections Using Primary Feline Kidney Cells

Setup of Aerosol Delivery System to Prevent Measles Virus Infection in Nonhuman Primate Model

Histopathological Analysis of Brains from Dogs Infected with Canine Distemper Virus

Canine Distemper Virus Pathogenesis in the Ferret Model

Measles Foci Reduction Neutralization Test (FRNT)

Multiplex Bead Assay for Serological Surveillance of Measles and Rubella

Measles IgG Avidity Assay.

ISBN:

9781071638705