Protein Tyrosine Phosphatases : Methods and Protocols / edited by Damien Thévenin, Jörg P. Müller.

Format:

Book

Contributor:

Thévenin, Damien., Editor.

P. Müller, Jörg., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2743

Language:

English

Subjects (All):

Proteins.

Cytology.

Cell Biology.

Local Subjects:

Proteins.

Cell Biology.

Physical Description:

1 online resource (XI, 318 p. 135 illus., 47 illus. in color.)

Edition:

2nd ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This second edition volume expands on the previous edition with discussions on the latest advancements in protein tyrosine phosphatases (PTP) research used to investigate these essential enzymes and new inhibitors. The new techniques covered in the chapters of this book include studying enzymes in vitro, in cells, and in animal models through proteomics, genomics, and structural biology. Furthermore, new advances in pharmacology and drug design have contributed to the developing novel therapeutics that target PTPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Protein Tyrosine Phosphatases: Methods and Protocols, Second Edition is a valuable resource for both experienced andnovel researchers in this field, and will lead to discoveries and accelerated progress in the field of PTP, signal transduction, and drug development. .

Contents:

Induction of Translational Readthrough on Protein Tyrosine Phosphatases Targeted by Premature Termination Codon Mutations in Human Disease

Understanding Pseudophosphatase Function through Biochemical Interactions

CRISPR/Cas9-Mediated Modification of PTP Expression

Osteoclast Methods in Protein Phosphatase Research

OT-I TCR Transgenic Mice to Study the Role of PTPN22 in Anti-Cancer Immunity

Protein-Tyrosine Phosphatases Studied in Zebrafish

Examining Phosphatases through Immunofluorescent Microscopy

Identification of Protein Tyrosine Phosphatase (PTP) Substrates

Kinase-Catalyzed Biotinylation to Identify Phosphatase Substrates (K-BIPS)

Systems-Level Analysis of the Effects of RPTPs on Cellular Signaling Networks

Detection of Protein Tyrosine Phosphatase Interacting Partners by Mass Spectrometry

Detecting PTP Protein-Protein Interactions by Fluorescent Immunoprecipitation Analysis (FIPA)

Identifying Transmembrane Interactions in Receptor Protein Tyrosine Phosphatase Homodimerization and Heterodimerization

Preparation of Oxidized and Reduced PTP4A1 for Structural and Functional Studies

Measuring the Reversible Oxidation of Protein Tyrosine Phosphatases using a Modified Cysteinyl-Labeling Assay

Identification and Optimization of Protein Tyrosine Phosphatase Inhibitors via Fragment Ligation

Targeting Non-Conserved and Pathogenic Cysteines of Protein Tyrosine Phosphatases with Small Molecules

In Vitro Phosphatase Assays for the Eya2 Tyrosine Phosphatase

High Throughput Discovery and Characterization of Covalent Inhibitors for Protein Tyrosine Phosphatases.

ISBN:

9781071635698

1071635697