Genotype Phenotype Coupling : Methods and Protocols / edited by Stefan Zielonka, Simon Krah.

Format:

Book

Contributor:

Zielonka, Stefan., Editor.

Krah, Simon., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2681

Language:

English

Subjects (All):

Biology--Technique.

Biology.

Experimental immunology.

Genetics.

Biologicals.

Biopharmaceutics.

Immunological Techniques.

Genotype.

Biologics.

Local Subjects:

Immunological Techniques.

Genotype.

Biologics.

Physical Description:

1 online resource (XV, 408 p. 75 illus., 65 illus. in color.)

Edition:

2nd ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics and diagnostic reagents in a shorter time frame. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genotype Phenotype Coupling: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to expand their knowledge of antibody-based therapeutics.

Contents:

Quantitative Determination of Staphylococcus aureus Using Aptamer-Based Recognition and DNA Amplification Machinery

Construction of Synthetic VHH Libraries in Ribosome Display Format

Isolation of Adhirons Specific for Plant Protoplast Membrane Biomarkers is Simplified by Phagemid Design

Facile One-Step Generation of Camelid VHH and Avian scFv Libraries for Phage Display by Golden Gate Cloning

Identification of New Antibodies Targeting Tumor Cell Surface Antigens by Phage Display

Phage Display of Bovine Ultralong CDRH3

Bacterial Cell Display for Selection of Affibody Molecules

Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display

Selection of High-Affinity Heterodimeric Antigen-Binding Fc Fragments from a Large Yeast Display Library

A Two-Step Golden Gate Cloning Procedure for the Generation of Natively Paired YSD Fab Libraries

Single-Cell B-Cell Sequencing to Generate Natively-Paired scFab Yeast Surface Display Libraries

One-Pot Droplet RT-OE-PCR for the Generation of Natively Paired Antibody Immune Libraries

Affinity Maturation of the Natural Ligand (B7-H6) for Natural Cytotoxicity Receptor NKp30 by Yeast Surface Display

Accessing Transient Binding Pockets by Protein Engineering and Yeast Surface Display Screening

Tyrosine Phosphorylation Screening on the Yeast Surface by Magnetic Bead Selection and FACS

Bulk Reformatting of Antibody Fragments Displayed on the Surface of Yeast Cells to Final IgG Format for Mammalian Production

Antibody Secreting Cell Isolation from Different Species for Microfluidic Antibody Hit Discovery

Efficient Microfluidics Downstream Processes for Rapid Antibody Hit Confirmation

Cell Line Development Using Targeted Gene Integration into MAR-Rich Landing Pads for Stable Expression of Transgenes

Generation of Human 293-F Suspension NGFR Knockout Cells Using CRISPR/Cas9 Coupled to Fluorescent Protein Expression

Antibody Display Technology (ADbody) to Present Challenging and Unstable Target Proteins on Antibodies

SUMO: In Silico Sequence Assessment Using Multiple Optimization Parameters

Streamlined Data Analysis Pipeline for Deep Sequence-Coupled Biopanning Identification of Pathogen-Specific Antibody Responses in Serum.

ISBN:

9781071632796

1071632795