Pyroptosis : Methods and Protocols / edited by Susan L. Fink.

Format:

Book

Contributor:

Fink, Susan L., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2641

Language:

English

Subjects (All):

Cytology.

Cell death.

Cell Biology.

Cell Death.

Local Subjects:

Cell Biology.

Cell Death.

Physical Description:

1 online resource (XI, 224 p. 36 illus., 30 illus. in color.)

Edition:

1st ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This volume explores techniques used to study and understand the latest research on pyroptosis. The chapters in this book cover topics such as experimental methods to induce pyroptosis via inflammasomes; approaches to identifying inflammasome activation in humans; ways to induce and detect pore formation; and techniques to evaluate cellular outcomes of pyroptotic cell death. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Pyroptosis: Methods and Protocols is a valuable tool for researchers who want to expand their knowledge of this developing field. It will be especially beneficial for those working in the fields of immunology, cancer biology, microbiology, and cell biology.

Contents:

Molecular Mechanisms of Pyroptosis

Assessment of NLRP3 Inflammasome Activation and NLRP3-NEK7 Complex Assembly

Inducing Pyroptosis with FlaTox, RodTox, or NeedleTox

Inducing Pyroptosis via the Pyrin Inflammasome

Caspase-4 Activation and Recruitment to Intracellular Gram-Negative Bacteria

CARD8 Inflammasome Activation by HIV-1 Protease

Pyroptosis Markers in Human Primary Specimens: Quantification of Intracellular ASC Specks by Imaging Flow Cytometry and Extracellular Oxidized Mitochondrial by ELISA

Identifying Inflammasome Activation in Human Tissues

Analyzing Caspase-8-Dependent GSDMD Cleavage in Response to Yersinia Infection

Biochemical Methods for Assessing Gasdermin D Inactivation in Macrophages

Detection of Gasdermin C-Mediated Cancer Cell Pyroptosis

Inducing Pyroptosis via Gasdermin B and Gasdermin E Cleavage

Assessing Interleukin-1β Activation during Pyroptosis

Monitoring Calcium Fluxes and Lysosome Exocytosis during Pyroptosis

Assessing Cytoskeletal Destruction During Pyroptosis

Assessment of HMGB1 Release During Experimentally-Induced Pyroptosis

Time-Lapse Imaging of Inflammasome-Dependent Cell Death and Extrusion in Enteroid-Derived Intestinal Epithelial Monolayers.

ISBN:

9781071630402

1071630407