Chemogenomics : Methods and Protocols / edited by Daniel Merk, Apirat Chaikuad.

Format:

Book

Contributor:

Merk, Daniel., Editor.

Chaikuad, Apirat., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2706

Language:

English

Subjects (All):

Biochemical genetics.

Pharmacology.

Chemical Genetics.

Local Subjects:

Chemical Genetics.

Pharmacology.

Physical Description:

1 online resource (XI, 234 p. 62 illus., 60 illus. in color.)

Edition:

1st ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This volume presents both theoretical guidance and protocols on chemogenomics including chemogenomics library assembly, compound profiling, and phenotypic assays. The chapters in this book cover topics such as the assembly and use of Kinase Chemogenomics; data mining for chemogenomic compound candidates; protocols for protein family-focused assay systems to profile chemogenomic compounds; functional and target engagement assays in cellular settings for broad characterization; and a discussion on phenotypic assays where chemogenomic sets may be applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Chemogenomics: Methods and Protocols is a valuable resource for all researchers whoare interested in learning more about this diverse and developing field. .

Contents:

An Introduction to Chemogenomics

Developing a Kinase Chemogenomic Set: Facilitating Investigation into Kinase Biology by Linking Phenotypes to Targets

Compilation of Custom Compound/Bioactivity Datasets from Public Repositories

Quality Control of Chemogenomic Library using LC-MS

Annotation of the Effect of Chemogenomic Compounds on Cell Health using High-Content Microscopy in Live-Cell Mode

Characterization of Cellular Viability using Label-Free Brightfield Live-Cell Imaging

Plate-Based Screening for DUB Inhibitors

NanoBRET™ Live Cell Kinase Selectivity Profiling Adapted for High Throughput Screening

A Fluorescence-Based Reporter Gene Assay to Characterize Nuclear Receptor Modulators

Measuring Protein-Protein Interactions in Cells using NanoLuciferase Bioluminescence Resonance Energy Transfer (NanoBRET) Assay

HiBiT Cellular Thermal Shift Assay (HiBiT CETSA)

Detection of Cellular Target Engagement for Small Molecule Modulators of Striatal-Enriched Protein Tyrosine Phosphatase (STEP)

Target Deconvolution by Limited Proteolysis Coupled to Mass Spectrometry

Global Assessment of Drug Target Engagement and Selectivity of Covalent Cysteine-Reactive Inhibitors using Alkyne-Functionalized Probes

3D-Spheroid Invasion Assay for High-Throughput Screening of Small Molecule Libraries

Live-Cell High-Throughput Screen for Monitoring Autophagy Flux

Phenotypic Chemical Screening in CD4+ T Cells to Identify Epigenetic Inhibitors.

ISBN:

9781071633977

107163397X