Forensic DNA Analysis : Methods and Protocols / edited by Catherine Cupples Connon.

Format:

Book

Contributor:

Cupples Connon, Catherine., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2685

Language:

English

Subjects (All):

Genetics.

Medical jurisprudence.

Genetics and Genomics.

Forensic Medicine.

Local Subjects:

Genetics and Genomics.

Forensic Medicine.

Physical Description:

1 online resource (IX, 431 p. 95 illus., 92 illus. in color.)

Edition:

1st ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchersinterested in learning more about forensic DNA analysis procedures.

Contents:

Forensic DNA Analysis: An Overview of the Laboratory Process

Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods

Manual Silica-Based DNA Extractions

Applied Biosystems™ PrepFiler™ Forensic DNA Extraction Kit (Manual and Semi-Automated via AutoMate Express™)

Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation

DNA Extraction of Bone through Demineralization

Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ™ Methods

DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards

Yield Gel via Quantitative Gel Electrophoresis

Quantitative PCR of Alu Repeats using PowerUp™ SYBR® Green Master Mix

Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit

QIAGEN's Investigator® Quantiplex Pro Kit

DNA Amplification using Promega's PowerPlex® Fusion Systems (5C and 6C)

Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System

Applied Biosystems' GlobalFiler™ PCR Amplification Kit

QIAGEN’s Investigator® 24plex QS and GO! PCR Amplification

Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis

Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer

Likelihood Ratio Calculation using LRmix Studio

Mitochondrial DNA Analysis

An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints

Detection of Latent DNA using a DNA Binding Dye

Rapid DNA Profile Development with Applied Biosystems RapidHIT™ ID System

Next-Generation Sequencing: ForenSeq™ DNA Signature Prep Kit with the Illumina MiSeq FGx.

ISBN:

9781071632956

1071632957

1-07-163295-7