Teratogenicity Testing : Methods and Protocols / edited by Luís Félix.

Format:

Book

Contributor:

Félix, Luís., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2753

Language:

English

Subjects (All):

Developmental biology.

Toxicology.

Embryology.

Developmental Biology and Stem Cells.

Medical Toxicology.

Local Subjects:

Developmental Biology and Stem Cells.

Medical Toxicology.

Embryology.

Physical Description:

1 online resource (XVI, 627 p. 113 illus., 96 illus. in color.)

Edition:

2nd ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This fully updated volume collects laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. Beginning with a section detailing basic principles of teratogenicity, the book continues with application of in vitro models for teratogenic screening to predict toxicity in embryonic animals and humans, as well as a variety of well-established in vivo animal model tests, which offer solutions for evaluating the efficacy and the teratogenicity of compounds in preclinical trials before human safety testing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Teratogenicity Testing: Methods and Protocols, Second Edition serves as an ideal guide to tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.

Contents:

Updating an Overview of Teratology

Guidelines on Development Toxicity Tests: Brief Insights

Development Features on the Selection of Animal Models for Teratogenic Testing

Virus as Teratogenic Agents

The Future of the Teratogenicity Testing

The First Steps on AOPs' Concepts, Development, and Applications in Teratology

Machine Learning to Predict Teratogenicity: Theory and Practice

Human Pluripotent Stem Cell-Based Assays to Predict Developmental Toxicity

Developmental Toxicity Using the Rat Whole Embryo Culture

A Modified Murine Embryonic Stem Cell Test for Evaluating the Teratogenic Effects of Drugs

Teratogenic Effects of Drugs on Primary Lymphocytes Assessed by Flow Cytometry

Assessment of the Teratogenic Effect of Drugs on the Chicken Embryo

Daphnia magna as a Model Organism to Predict the Teratogenic Effect of Different Compounds

Caenorhabditis elegans as an In Vivo Model Organism to Elucidate Teratogenic Effects

Enduring Ethanol-Induced Behavioral Alterations in Caenorhabditis elegans after Developmental Lead Exposure

Exploration of Teratogenic and Genotoxic Effects on Model Organism Drosophila melanogaster

Ecotoxicological Assessment of Seaweed-Based Crop Biostimulant on Earthworm Eudrilus eugeniae Kinb

Evaluation of the Ecotoxicology of Seaweed-Based Biopesticide Used in Combat of the Polyphagous Pest Using Eudrilus eugeniae Kinb

Biochemical Studies to Understand Teratogenicity and Lethality Outcomes in Modified-FETAX

Bioinformatics Methods for Transcriptome Analysis on Teratogenesis Testing

Angiogenesis Assay for Live and Fixed Zebrafish Larvae

High-Resolution Respirometry for the Assessment of Teratogenic Chemicals

A Stereological Approach to Quantify Immunohistochemical Staining in Zebrafish Larvae

Whole-Mount Immunohistochemical and Immunofluorescence Assays in Zebrafish Embryos

Assessment of Developmental Neurotoxicity Using Semi-Automatic Behavior Analysis System for Zebrafish

Behavioral Profiling of Zebrafish (Danio rerio) Larvae: Activity, Anxiety, Avoidance, and Startle Response

Alcian Blue Staining for Chondrocranium Development in Zebrafish

Protocol of Geometric Morphometrics for Teratogenicity Testing

Biochemical Markers for Liver Injury in Zebrafish Larvae

Cortisol Quantification for Assessing Stress-Induced Changes in Zebrafish Larvae

Metabolomic Fingerprint Assay in Zebrafish Embryos

Alkaline Comet Assay to Assess Genotoxicity in Zebrafish Larvae

A Chromogenic Quantification of Protein Expression in Zebrafish Larvae

Confocal Microscopy Technique in Teratogenicity Testing Using Zebrafish (Danio rerio) Embryos as Model

Whole-Mount RNA In Situ Hybridization of Zebrafish Embryos

Quantitative Real-Time PCR Method to Evaluate Gene Expression in Zebrafish Embryos

Microarray Analysis to Determine Gene Expression Changes in Zebrafish Embryos

Cell-Free DNA and Next-GenerationSequencing for Prenatal Diagnosis.

ISBN:

9781071636251

1071636251