Flower Development : Methods and Protocols / edited by José Luis Riechmann, Cristina Ferrándiz.

Format:

Book

Contributor:

Riechmann, José Luis, Editor.

Ferrándiz, Cristina, Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2686

Language:

English

Subjects (All):

Botany.

Plant Science.

Local Subjects:

Plant Science.

Physical Description:

1 online resource (XV, 588 p. 86 illus., 67 illus. in color.)

Edition:

2nd ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Flower Development: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .

Contents:

Flower development in Arabidopsis

Flower development in the Solanaceae

The ABC of flower development in monocots: the model of rice spikelet

Model species to investigate the origin of flowers

Hormones and flower development in Arabidopsis

Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors

Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development

Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and rice

Isolation of meiocytes and cytological analyses of male meiotic chromosomes in soybean, lettuce and maize

Genetic and phenotypic analyses of carpel development in Arabidopsis

Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis

Floral induction systems for the study of Arabidopsis flower development

Protoplasting and Fluorescence-activated cell sorting of the shoot apical meristem cell types

Protoplast Isolation for Plant Single-Cell RNA-seq

Plant Nuclei Isolation for Single-nucleus RNA Sequencing

Isolation of nuclei tagged in specific cell types (INTACT) in Arabidopsis

RNA in situ hybridization on plant tissue sections: expression analysis at cellular resolution

The GUS reporter system in flower development studies

Expression and functional studies of leaf, floral and fruit developmental genes in non-model species

Gene expression analysis by quantitative real-time PCR for floral tissues

Misexpression approaches for the manipulation of flower development

Genomic approaches for the study of flower development in floriculture crops

Multi-omics methods applied to flower development

Peptidomics methods applied to the study of flower development

Quantifying gene expression domains in plant shoot apical meristems

A NanoLuc-based transactivation assay in plants

A hands-on guide to generate spatial gene expression profiles by integrating scRNA-seq and 3D reconstructed microscope-based plant structures.

ISBN:

9781071632994

107163299X

1-07-163299-X