Phage Display : Methods and Protocols / edited by Michael Hust, Theam Soon Lim.

Format:

Book

Contributor:

Hust, Michael., Editor.

Lim, Theam Soon., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2702

Language:

English

Subjects (All):

Biology--Technique.

Biology.

Experimental immunology.

Immunological Techniques.

Local Subjects:

Immunological Techniques.

Physical Description:

1 online resource (XV, 605 p. 83 illus., 69 illus. in color.)

Edition:

2nd ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This second edition details new and updated methods on different antibody libraries, along with novel approaches for antibody discovery. Chapters focuses on the construction of antibody libraries, antibody expression, complementary approaches for antibody selection, and other phage display related applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phage Display: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .

Contents:

Antibody phage display

Construction of human immune and naive scFv Phage Display libraries

Construction of Naïve and Immune Human Fab Phage Display Library

Construction of synthetic antibody phage display libraries

Construction of chicken and ostrich antibody libraries

Construction of Rabbit Immune Antibody Libraries

Isolation and characterization of single-domain antibodies from immune phage display libraries

Phagekines: directed evolution and characterization of functional cytokines displayed on phages

Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly

Construction of an ultra-large phage display library by Kunkel mutagenesis and rolling circle amplification

Construction of semi-synthetic shark vNAR yeast surface display antibody libraries

Antibody selection via Phage Display in microtiter plates

Antibody selection in solution using magnetic beads

Streptavidin Coated Solid Phase Extraction (SPE) TipsFor Antibody Phage Display Biopanning

Magnetic Nanoparticle-Based Semi-Automated Panning for High-throughput Antibody Selection

Antibody selection on cells targeting membrane proteinsTargeting Intracellular Antigens With pMHC-binding Antibodies: A Phage Display Approach

Antibody Isolation from a Human Synthetic Libraries of Single-chain Antibodies and analysis using NGS

Selection of affibody affinity proteins from phagemid libraries

Antibody affinity and stability maturation by error-prone PCR

Antibody batch cloning

Deep Mining of Complex Antibody Phage Pools

High-throughput IgG reformatting and expression using hybrid secretion signals and InTag positive selection technology

Validation and the Determination of Antibody Bioactivity Using MILKSHAKE and Sundae Protocols

Mapping polyclonal antibody responses to infection using next generation phage display

Applications of high-throughput DNA sequencing to single-domain antibodydiscovery and engineering

Biomarker discovery by ORFeome Phage Display

Mapping Epitopes by Phage Display

Epivolve: A Protocol for Site-directed Antibodies.

ISBN:

9781071633816

1071633813