Neuroprotection : Method and Protocols / edited by Swapan K. Ray.

Format:

Book

Contributor:

Ray, Swapan K., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2761

Language:

English

Subjects (All):

Neurosciences.

Neuroscience.

Local Subjects:

Neuroscience.

Physical Description:

1 online resource (XIX, 641 p. 160 illus., 123 illus. in color.)

Edition:

1st ed. 2024.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2024.

Summary:

This volume contains cutting-edge molecular biology methods on neuroprotective mechanisms and specific preclinical models of the CNS injury, iseases and planning translation. Chapters guide readers through neuropathology, neuroprotection, Alzheimer’s disease, amyotrophic lateral sclerosis, ALS, Huntington’s disease , multiple sclerosis, Parkinson’s disease, spinal cord injury, traumatic brain injury, and ischemic brain injury. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neuroprotection: Methods and Protocols aims to ensure successful results in the further study of this vital field. .

Contents:

TUNEL-n-DIFL Method for Detection and Estimation of Apoptosis Specifically in Neurons and Glial Cells in Mixed Culture and Animal Models of Central Nervous System Diseases and Injuries

Isolation of Capillaries from Small Amounts of Mouse Brain Tissue

Isolation of Extracellular Vesicles Using Formulas to Adapt Centrifugation to Different Centrifuges

High-resolution Respirometry for Mitochondrial Function in Rodent Brain

Quantification of Neuronal Dendritic Spine Density and Lengths of Apical and Basal Dendrites in Temporal Lobe Structures Using Golgi-Cox Staining

Quantification of Neuroinflammatory Markers in Blood, Cerebrospinal Fluid, and Resected Brain Samples Obtained from Patients

Targeted Modification of Epigenetic Marks Using CRISPR/Dcas9-Suntag-based Modular Epigenetic Toolkit

Elevated Plus Maze for Assessment of Anxiety and Memory in Rodents

Drosophila Neuromuscular Junction as a Model to Study Synaptopathies and Neuronal Autophagy

Cell-based Assay to Detect the Autoantibody Serostatus in Patients with Neuromyelitis Optica Spectrum Disorder (NMOSD)

Using Small Molecules for Targeting Heavy Metals in Neurotoxicity and Neuroinflammation

Chromatographic Separation and Quantitation of Sphingolipids from the Central Nervous System or Any Other Biological Tissue

Role of Network Pharmacology in Prediction of Mechanism of Neuroprotective Compounds

Role of Serotonergic System in Regulating Brain Tumor-associated Neuroinflammatory Responses

Impacts of Omega-3 Fatty Acids, Natural Elixirs for Neuronal Health, on Brain Development and Functions

Microglial Uptake of Extracellular Tau by Actin-mediated Phagocytosis

Internalization and Endosomal Trafficking of Extracellular Tau in Microglia Improved by α-Linolenic Acid

Understanding Actin Remodeling in Neuronal Cells Through Podosomes

Quantitative Investigation of Neuroprotective Role of ROR1in a Cell Culture Model of Alzheimer's Disease

microRNA Isolation, Expression Profiling, and Target Identification for Neuroprotection in Alzheimer's Disease

Quantitative Measurement of Tau Aggregation in Genetically Modified Rats with Neurodegeneration

Detection and Characterization of Apoptosis-related Proteins in Hippocampal Neurodegeneration: From mRNA Expression to Protein Quantification

Isolation and Detection of Pathological Tau Species in a Tauopathy Mouse Model

Enzyme Inhibition Assays for Monoamine Oxidase

Role of Amyloid Beta in Neurodegeneration and Therapeutic Strategies for Neuroprotection

Amyloid Beta Mediated Neurovascular Toxicity in Alzheimer’s Disease

Fecal Microbiota Transplantation in Amyotrophic Lateral Sclerosis: Clinical Protocol and Evaluation of Microbiota Immunity Axis

Integrated Multi-omics Analysis and Validation in Yeast Model of Amyotrophic Lateral Sclerosis

Imaging and Assay of the Dynamics of Cytotoxic Huntingtin (HTT) Protein Aggregates Regulated by lncRNAs

Astrocyte Activation and Drug Target in Pathophysiology of Multiple Sclerosis

Promises of Lipid-based Nanocarriers for Delivery of Dimethyl Fumarate to Multiple Sclerosis Brain

Chrysin for Neurotrophic and Neurotransmitter Balance in PD

Establishment of a 6-OHDA Unilateral Lesion Male Wistar Rat Model of Parkinson's Disease

Evaluating Motor Dysfunction and Oxidative Stress Induced by Trichloroethylene in Wistar Rats

Neurobehavioral Analysis to Assess Olfactory and Motor Dysfunction in Parkinson’s Disease

Ion Channels and Metal Ions in Parkinson’s Disease: Historical Perspective to the Current Scenario

Creating a Reproducible Model of Spinal Cord Injury in Rats: A Contusion Approach

Weight Drop Method for Inducing Closed Head Diffuse Traumatic Brain Injury

iDISCO Tissue Clearing Whole-Brain and Light Sheet Microscopy for High-ThroughputImaging in a Mouse Model of Traumatic Brain Injury

Insights From Rodent Models for Improving Bench-to-Bedside Translation in Traumatic Brain Injury

Rat Model of Middle Cerebral Artery Occlusion.

ISBN:

9781071636626

1071636626