Toll-Like Receptors : Methods and Protocols / edited by Francesca Fallarino, Marco Gargaro, Giorgia Manni.

Format:

Book

Contributor:

Fallarino, Francesca., Editor.

Gargaro, Marco., Editor.

Manni, Giorgia., Editor.

Series:

Methods in Molecular Biology, 1940-6029 ; 2700

Language:

English

Subjects (All):

Immunology.

Cancer.

Cancers.

Local Subjects:

Immunology.

Cancers.

Physical Description:

1 online resource (XI, 286 p. 45 illus., 42 illus. in color.)

Edition:

1st ed. 2023.

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

Summary:

This volume provides methods on the role of TLRs in immune responses and on the various approaches targeting different TLRs in cancer, autoimmunity and in vaccine development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Toll-Like Receptors: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .

Contents:

Modelling of transmembrane domain and full length TLRs in membrane models

Development of Optimal Virtual Screening Strategies to Identify Novel Toll-like Receptor Ligands using the DockBox Suite

Use of Fluorescent Chemical Probes in the Study of Toll-like Receptors (TLRs) Trafficking

Endotoxin-tolerance mimicking to study TLR in promotion of tolerogenic DCs and Tr1 cells

Flow cytometry analysis of IL-1 receptors and Toll like receptors on platelets and platelet-derived extracellular vesicles

Methods to study TLRs in transplantation

Determining endosomal Toll-like Receptors gene expression in NK cells after stimulation with specific agonists

In vitro study of TLR4-NLRP3-inflammasome activation pathway in innate immune response

Toll-like receptor polymorphisms and the risk of cancer: meta-analysis study

Unrevealing the role of TLRs in the pathogenesis of autoimmune disease by using mouse model of diabetes

In vitro and ex vivo methodologies for T cell trafficking through Blood Brain Barrier after TLR activation

Delineating the role of Toll-like receptors in inflammatory bowel disease

Analysis of differential TLR activation in a mouse model of multiple sclerosis

Study of agonists of TLRs as vaccine adjuvants

Activation of TLRs by opportunistic fungi in lung organoids. .

ISBN:

9781071633663

107163366X