Polypeptide Functionalized Gold Nanoparticles for Bioanalytical Applications.

Format:

Book

Author/Creator:

Selegård, Robert.

Series:

Linköping Studies in Science and Technology. Dissertations

Linköping Studies in Science and Technology. Dissertations ; v.1598

Language:

English

Subjects (All):

Biosensors.

Metal nanoparticles.

Physical Description:

1 online resource (79 pages)

Edition:

1st ed.

Other Title:

Linköping Studies in Science and Technology. Dissertations

Place of Publication:

Linköping : Linköping University Electronic Press, 2014.

Summary:

This dissertation by Robert Selegård explores the development and application of polypeptide gold nanoparticles for bioanalytical purposes. The research focuses on innovative strategies for protein affinity sensing and the detection of critical biological processes using gold nanoparticles coupled with a synthetic polypeptide. The study highlights the tunable self-assembly of these nanoparticles, driven by Zinc ion interactions, and their potential in creating cost-effective, sensitive detection methods for proteins. The work offers significant advancements in drug development and diagnostics, particularly through the development of novel colorimetric biosensing techniques. It is intended for researchers and professionals in the fields of molecular physics, chemistry, and bioanalytical technology. Generated by AI.

Contents:

Intro

ABSTRACT

TACK

LIST OF PUBLICATIONS

AUTHOR CONTRIBUTIONS

PUBLICATION NOT INCLUDED IN THIS THESIS

ABBREVIATIONS

AMINO ACIDS

CONTENTS

CHAPTER 1

Introduction

Bionanotechnology and Self-Assembly

2.1 Supramolecular chemistry

2.2 Molecular self-assembly

2.3 Nanotechnology and bionanotechnology

2.4 Nanocomposites and hybrid nanomaterials

CHAPTER 3

Structure of Proteins and Protein Folding

3.1 Protein folding

3.2 Circular dichroism spectroscopy

CHAPTER 4

Synthetic Peptides

4.1 JR2EC

4.2 KE2C

4.3 Peptide synthesis

4.3.1 Peptide purification

4.3.2 Peptide identification

4.4 Peptide functionalization

4.4.1 Click chemistry

CHAPTER 5

Metal Cations and Biomolecules

5.1 Metalloproteins

5.2 Attributes of zinc(II)

5.3 Zinc(II) - biomolecule interactions

CHAPTER 6

Colloidal Gold

6.1 Gold nanoparticle synthesis

6.2 Optical properties of gold nanoparticles

6.3 Colloidal stability

6.3.1 DLVO forces

6.3.2 Non-DLVO forces

6.4 Gold nanoparticles stabilized by JR2EC

CHAPTER 7

Polypeptide Functionalized Gold Nanoparticles

7.1 Immobilization of JR2EC onto gold nanoparticles

7.2 Zinc(II) induced aggregation

CHAPTER 8

Gold Nanoparticles in Bioanalytical Applications

8.1 Colorimetric assays

8.2 Colorimetric protein sensing

8.3 Colorimetric protease sensing

8.4 Colorimetric zinc-protein-chelant sensing

8.5 Colorimetric phosphatase sensing

CHAPTER 9

Summary of Papers

CHAPTER 10

Future Outlook

CHAPTER 11

References.

Notes:

Description based on publisher supplied metadata and other sources.

Part of the metadata in this record was created by AI, based on the text of the resource.

ISBN:

9789175193212

9175193213

OCLC:

927227310