Methods in Biotechnology.

Format:

Book

Author/Creator:

Hong, Seung-Beom.

Contributor:

Rashid, M. Bazlur.

Santiago-Vazquez, Lory Z.

Language:

English

Subjects (All):

Biotechnology--Laboratory manuals.

Physical Description:

1 online resource (722 pages)

Edition:

1st ed.

Place of Publication:

New York : John Wiley & Sons, Incorporated, 2016.

Summary:

As rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods; Methods in Biotechnology is an invaluable resource for those students and professionals. Methods in Biotechnology engages the reader by implementing an active learning approach, provided advanced study questions, as well as pre- and post-lab questions for each lab protocol. These self-directed study sections encourage the reader to not just perform experiments but to engage with the material on a higher level, utilizing critical thinking and troubleshooting skills. This text is broken into three sections based on level - Methods in Biotechnology, Advanced Methods in Biotechnology I, and Advanced Methods in Biotechnology II. Each section contains 14-22 lab exercises, with instructor notes in appendices as well as an answer guide as a part of the book companion site. This text will be an excellent resource for both students and laboratory professionals in the biotechnology field.

Contents:

Intro

Title Page

Copyright

Table of Contents

Preface

Acknowledgements

Introduction to biotechnology lab

Lab safety

Good work habits

Lab etiquette

Lab record

Lab report and assignment

Further reading

Laboratory Schedule (Methods in Biotechnology (MB))

Laboratory Schedule (Advanced Methods in Biotechnology (AMB) 1)

Laboratory Schedule (AMB 2)

About the companion website

Part I: Methods in Biotechnology (MB) Laboratory Exercises

Chapter 1: MB experiment 1: Lab measurements

Introduction

Pre-lab assignment

Materials and equipment

Procedure

Part I. Pipetting skill

Part II. Density and specific gravity

Discussion

Post-lab assignment

Chapter 2: MB experiment 2: Use of the spectrophotometer and Beer's law

Result

Chapter 3: MB experiment 3: Making solutions and buffer efficacy

Part I. pH meter calibration

Part II. Making solutions (to share four groups)

Part III. Buffering capacity (addition of strong acid and strong base to buffered and unbuffered solutions)

Part IV. Dilution effect on buffer pH and buffer capacity

Chapter 4: MB experiment 4: Acid-base titration

Part I. Two-point calibration of pH Meter

Part II. Titration of acetic acid with NaOH

Part III. Titration of HCl with NaOH

Part IV. Data integration

Chapter 5: MB experiment 5: Protein denaturation and precipitation

Pre-lab assignment.

Materials and equipment

Chapter 6: MB experiment 6: Bacterial transformation

Procedure (day 1)

Procedure (day 2)

Procedure (day 3)

Chapter 7: MB experiment 7: GFP purification

Chapter 8: MB experiment 8: SDS-PAGE analysis

Chapter 9: MB experiment 9: DNA isolation

Part I. Plasmid DNA isolation

Part II. Genomic DNA isolation

Chapter 10: MB experiment 10: PCR-based Alu-human DNA typing

Part E. Viewing and photography

Chapter 11: MB experiment 11: Restriction enzyme digestion

Chapter 12: MB experiment 12: Agarose gel electrophoresis

Chapter 13: MB experiment 13: Ouchterlony and ELISA immunoassays

Part I. Ouchterlony (double diffusion) assay

Part II. ELISA assay

Further reading.

Chapter 14: MB experiment 14: Testing plant substances for antimicrobial activity

Chapter 15: MB experiment 15: Peroxidase enzyme activity assay

Results

References

Part II: Advanced Methods in Biotechnology (AMB) 1 Laboratory Exercises

chapter 16: AMB 1 experiment 16: Aseptic technique and culture handling

chapter 17: AMB 1 experiment 17: Yeast culture media preparation

chapter 18: AMB 1 experiment 18: Growth curve

Chapter 19: AMB 1 experiment 19: Mini plasmid prep

Procedure (adapted from QIAprep miniprep kit)

Additional information

Chapter 20: AMB 1 experiment 20: Restriction digestion, purification, concentration, and quantification of DNA

Chapter 21: AMB 1 experiment 21: Polymerase chain reaction (PCR)

Additional background information

Chapter 22: AMB 1 experiment 22: TA, blunt end, SLIC, and CPEC cloning of PCR product

Introduction.

Pre-lab assignment

Chapter 23: AMB 1 experiment 23: One-step multifragment assembly cloning

J. Screening of transformants

Chapter 24: AMB 1 experiment 24: Restriction enzyme digestion and fast agarose gel electrophoresis

chapter 25: AMB 1 experiment 25: Southern blot transfer

Reference

Chapter 26: AMB 1 experiment 26: Probe labeling and purification

Chapter 27: AMB 1 experiment 27: Dot blot assay

Chapter 28: AMB 1 experiment 28: Pre-hybridization, hybridization, and detection

Chapter 29: AMB 1 experiment 29: Total yeast RNA isolation and RT-PCR

Procedure (adapted from SV total isolation kit, Promega and verso one-step RT PCR Kit, Life technologies)

Chapter 30: AMB 1 experiment 30: Yeast-based in vivo recombination cloning

Part I. Isolation of overlapping DNA fragments (day 1)

Part II. Yeast transformation (day 2)

Chapter 31: AMB 1 experiment 31: Plasmid DNA isolation from yeast

Chapter 32: AMB 1 experiment 32: E. coli transformation with yeast plasmid DNA

Chapter 33: AMB 1 experiment 33: X-gal filter lift assay

Chapter 34: AMB 1 experiment 34: Protein quantitation assay

Part I. Bradford assay

Part II. Lowry assay

Part III. BCA assay

Material and equipment

Chapter 35: AMB 1 experiment 35: Quantitative β-Galactosidase assay in yeast

Part I. Permeabilized cell assay

Part II. Cell-free protein extract assay

Chapter 36: AMB 1 experiment 36: Gel filtration chromatography (GFC)

Chapter 37: AMB 1 experiment 37: Ion exchange chromatography (IEC)

Background

Part III: Advanced Methods in Biotechnology (AMB) 2 Laboratory Exercises

Chapter 38: AMB 2 experiment 38: E. coli culture media preparation

Procedure.

Post-lab assignment.

Notes:

Description based on publisher supplied metadata and other sources.

Other Format:

Print version: Hong, Seung-Beom Methods in Biotechnology

ISBN:

9781119156802

OCLC:

982011515