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Delta-Protocadherins Help Guide Olfactory Sensory Axons to Their Targets in the Olfactory Bulb / Daniel Barnes.
- Format:
- Book
- Thesis/Dissertation
- Author/Creator:
- Barnes, Daniel, author.
- Language:
- English
- Subjects (All):
- Neurosciences.
- Biology.
- Developmental biology.
- Biology--Penn dissertations.
- Penn dissertations--Biology.
- Local Subjects:
- Neurosciences.
- Biology.
- Developmental biology.
- Biology--Penn dissertations.
- Penn dissertations--Biology.
- Physical Description:
- 1 online resource (137 pages)
- Distribution:
- Ann Arbor : ProQuest Dissertations & Theses, 2023
- Contained In:
- Dissertations Abstracts International 85-08B.
- Place of Publication:
- [Philadelphia, Pennsylvania] : University of Pennsylvania, 2022.
- Language Note:
- English
- Summary:
- The olfactory system in zebrafish provides an ideal model to study the genes necessary for neural circuit formation. Olfactory sensory neurons (OSNs), born in the olfactory epithelium, project axons to the olfactory bulb. OSNs express a single odorant receptor from a repertoire of odorant receptor genes and OSNs expressing different odorant receptors are intermixed within the olfactory epithelium. OSN axons first target discrete, individually identifiable neuropilar regions called protoglomeruli that later in development are subdivided into smaller OR-specific glomeruli. OSNs expressing related ORs from OR homology clades A and B project to the central zone protoglomerulus, while OSNs expressing ORs from OR homology clade C project to the dorsal zone protoglomerulus. This suggests that there is coordinated expression of ORs and guidance genes in OSNs. 10x Genomics scRNA-seq experiments were performed to identify candidate guidance genes differentially expressed between OSNs targeting the dorsal zone and central zone protoglomeruli. Among the candidate guidance genes identified were four non-clustered protocadherins. The expression of pcdh11 and pcdh7b is enriched in OSNs targeting the dorsal zone protoglomerulus, while pcdh17 and pcdh10b expression is enriched in OSNs targeting the central zone protoglomerulus. The necessity of protocadherin expression in subsets of OSNs was assessed through CRISPR-Cas9 mediated F0 knockdown of each protocadherin. Both pcdh11 and pcdh7b were found to be necessary for OSNs to correctly target the dorsal zone protoglomerulus while pcdh17 and pcdh10b were each found to be necessary for OSNS to correctly target the central zone protoglomerulus. These findings demonstrate that subsets of OSNs express specific protocadherins that help determine their target locations in the olfactory bulb.
- Notes:
- Source: Dissertations Abstracts International, Volume: 85-08, Section: B.
- Advisors: Raper, Jonathan A.; Committee members: Kim, Junhyong; Granato, Michael; Bashaw, Greg J.; Luo, Wenqin.
- Department: Biology.
- Ph.D. University of Pennsylvania 2023.
- Local Notes:
- School code: 0175
- ISBN:
- 9798381510881
- Access Restriction:
- Restricted for use by site license.
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