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Regulation of Pre-Messenger RNA splicing / Douglas L. Black.

Henry Stewart Biomedical & Life Sciences Collection Available online

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Format:
Video
Author/Creator:
Black, Douglas L., author.
Language:
English
Subjects (All):
Eukaryotic cells.
Genetic transcription.
Physical Description:
1 streaming video file (31 min.) : sound, color
Place of Publication:
London, England : Henry Stewart Talks Ltd, 2007.
System Details:
video file
Contents:
The Regulation of Pre-messenger RNA Splicing
mRNAs are transcribed as precursor molecules
mRNA must undergo RNA processing reactions
Pre-mRNA splicing
The signals for splicing RNA are at the intron ends
Splicing proceeds by a two-step pathway
Splicing chemistry
Pre-mRNAs contain multiple exons and introns
The spliceosome
Structures of the spliceosomal snRNAs
The spliceosome assembles from snRNPs
The mature catalytic spliceosome
Minor class introns
Two features that affect splice site choice
The early choice of splice sites to pair
Additional controls on spliceosome assembly
A typical mammalian gene
Exon definition
SR proteins
SR proteins mediate the activity of ESEs
Mutations in splice sites
Human disease mutations that alter splicing
Alternative splicing can produce diverse proteins
Complex transcription units
The fibronectin gene
Patterns of alternative splicing
Alternative splicing generates protein diversity
The Drosophila DSCAM receptor
Gene number and complexity of the organism
More than ESE's
Somatic sexual development in the fruit fly
Sex-lethal is a sequence-specific RNA BP
The female Sxl protein regulates itself and Tra
Tra exon 2 has two possible 3' splice sites.
The upstream Tra 3' splice site
The female specific protein Tra is splicing regulator
Doublesex exon 4 contains a weak 3' splice site
The doublesex ESEs are dependent on Tra
Male and female mRNAs of Dsx produce TFs
Splicing regulation in vertebrates
Clusters of RNA elements
Many RNA binding proteins regulate splicing
Alternative splicing in the nervous system
The electrical properties of K+ channels
Cochlear hair cells
Some splicing regulators are tissue specific
Widely expressed splicing regulators
Problems for the future.
Notes:
Description based on publisher supplied metadata and other sources.
Retrieved April 10, 2024, from https://hstalks.com/bs/662/.

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