Stem cell memory / Prof. James Sherley.

Format:

Video

Author/Creator:

Sherley, James, author.

Series:

Henry Stewart talks

Language:

English

Subjects (All):

Stem cells.

Physical Description:

1 videorecording (01 hr., 03 min., 53 sec.) : sound, color.

Place of Publication:

London : Henry Stewart Talks Ltd, [2017]

System Details:

video file

Summary:

Audio-visual presentation : Specific and stable architecture of dynamic tissue cells ; the ability of stem cells to produce different cell types in a tissue while preserving their own identity ; asymmetric self renewal ; non-random chromosome segregation ; advances in the development of in-vitro and ex-vivo culture models for asymmetric self renewal and non-random chromosome segregation to investigate the molecular form of stem cell memory.

Contents:

Introduction

Part I: defining stem cell memory

Stem cells for biomedicine & biotechnology

Properties of two main stem cell types

Cellular memory

Embryonic stem cells lack cellular memory

Properties of "adult" stem cells

Tissue cell turnover

Non epithelial tissues similar kinetics & memory

Deterministic asymmetric self-renewal evidence

Hypotheses ASC asymmetric self-renewal

Evidence against asymmetric self-renewal

Evidence for asymmetric self-renewal

Asymmetric self-renewal debate

Two classes of adult stem cell memory

The genetic fidelity problem

Replication error mutation kinetics

Mutation avoidance: a deterministic possibility

A solution to the genetic fidelity problem

Chromosome segregation vs. co-segregation

In-vivo evidence for non-random co-segregation

Nonrandom segregation of sister chromatids

Immortal DNA strands in adult stem cells in vivo

Asymmetric self-renewal models

Immortal DNA strands

Co-segregation analysis

Florescent micrographs

Immortal DNA strands long term memory

General categories of possible mechanisms

Stable centrosome-microtubule-kinetochore

Centrosome-microtubule-kinetochore

Nonrandom co-segregation in budding yeasts

p53 expression in adult stem cells

Biochemical control of asymmetric self-renewal

Dissociation of non-random co-segregation

p53's role

Summary part I

Part II: potential molecular elements

Strategy

Engineered mouse cell models

ASRA gene signature discovery

85-gene ASRA signature

Properties of H2A.Z

Asymmetric detection of H2A.Z

Symmetric mitotic cells H2A.Z detection

Asymmetric mitotic cells H2A.Z detection

H2A.Z chromosomal asymmetry

Assay for detection of "H2A.Z asymmetry"

H2A.Z asymmetry in mouse hair follicle cells

Potential stem cell memory elements

Summary for part II.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 10, 2024, from https://hstalks.com/bs/123/.