RNAi and viral heart infections / Jens Kurreck.

Format:

Video

Author/Creator:

Kurreck, Jens, author.

Language:

English

Subjects (All):

Myocardium--Diseases.

Myocardium.

RNA interference.

RNA viruses.

Physical Description:

1 videorecording (51 min., 21 sec.) : sound, color

Place of Publication:

London : Henry Stewart Talks, 2007.

System Details:

video file

Contents:

Introduction

Overview - RNA interference as an antiviral tool

RNA interference pathway

RNA interference methods

Antisense vs. RNAi

Inhibition of HIV-1 by RNAi

Virus inhibition by RNAi - RNA viruses

Virus inhibition by RNAi - DNA viruses

Coxsackievirus B3 (CVB-3)

Picornavirus replication

Dilatated cardiomyopathy (DCM)

Overview - design of efficient siRNAs

siRNAs targeting the Coxsackievirus 5' UTR

siRNAs against 5' UTR of CVB-3 have no effect

Screening for efficient siRNAs against TRPV1

What makes an efficient siRNA?

Efficient siRNA - strand bias or target structure?

Accessible nucleotides at 3' or 5' end of target site

Activation of seemingly inactive siRNA

Folding of artificial siRNA targets

siRNA silencing and local stability - correlation

Model for determinants of siRNA efficiency

Overview - inhibition of coxsackievirus replication

Targeting the viral RdRP

Coxsackievirus replication in cell culture

Cell viability assay with siRdRP 1-4

Inhibition of virus replication by siRNA

Inhibition of Coxsackievirus B3 by siRNA

CVB-3 inhibition in vivo

Overview - strand selectivity of virus silencing

Replication cycle of picornaviruses

Selective strand silencing

Strand-selectivity of siRdRP

Design of siRNAs targeting the RdRP (-)-strand

Strand-specificity of silencing

Strand specific siRNAs

Strand-inactivation by incorporation of LNA

siRNA vs. siLNA

Overview - SiDEx

Silencing of RdRP by shRNA expression plasmid

Long-term silencing generates escape mutants

Silencing of mutated RdRP

SiRNA double expression vector (SiDEx)

SiDEx silences two target sites

SiDEx silences wt and mutated RdRP

SiDEx - silencing of mutated RdRP

Silencing of mutated target site 4

Overview - silencing of CAR

Coxsackievirus-adenovirus-receptor (CAR)

Screening for efficient siRNAs targeting CAR

Efficiency of CAR-siRNAs

Duration of silencing

Inhibition of virus propagation by CAR-siRNA

SiDEx vector against two target sites of CVB-3

Summary

Acknowledgement.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 8, 2024, from https://hstalks.com/bs/656/.