Small interfering RNA induced transcriptional gene silencing in human cells / Kevin V. Morris.

Format:

Video

Author/Creator:

Morris, Kevin V., author.

Language:

English

Subjects (All):

Genetic transcription.

Physical Description:

1 online resource (1 streaming video file (29 min., 09 sec.)) : sound, color

Place of Publication:

London, England : Henry Stewart Talks, 2007.

System Details:

video file

Contents:

Introduction

RNA interference (RNAi)

RNAi: Post-Transcriptional Gene Silencing (PTGS)

RNAi: Transcriptional Gene Silencing (TGS)

dsRNA mediated TGS in S. Pombe

Do siRNAs directed to promoters induce TGS?

FIV lentiviral vector system

Experimental protocol

siRNA targeting EF1a promoter suppress GFP

Specificity of EF1A siRNA Mediated TGS

siRNA does not alter vector integration

siRNA mediated TGS is reversible

TGS is associated with DNA methylation

siRNA EF52 can target the EF1A promoter

EF1a is only inhibited at 48hrs post-transfection

Are intronic regions susceptible to siRNA targeting

Intronic region is susceptible to siRNA targeting

Specificity of EF315 transcriptional silencing

siRNA mediated TGS in human cells

What is the mechanism of siRNA mediated TGS?

TGS operates via chromatin remodeling pathway

siRNA mediated histone methylation

RNA pol II is required for siRNA mediated TGS

How do siRNAs find the target?

Detection of flag-tagged DNMTs or Hp1s

Specificity of DNMT binding

Antisense siRNA targeting HIV-1 LTR/promoter

Susceptibility of the HIV-1 LTR to siRNA

siRNA EF52 and all the factors are linked

Conclusions

Putative mechanism(s) for siRNA mediated TGS

Acknowledgements

Living on the edge.

Notes:

Description based on: online resource; title from PDF information screen (Henry Stewart Talks Biomedical and Life Sciences Collection, viewed April 13 2024).

Retrieved April 13, 2024, from https://hstalks.com/bs/651/.