RNAi specificity : how big of an issue is it? / Peter J. Welch.

Format:

Video

Author/Creator:

Welch, Peter J., author.

Series:

Henry Stewart talks.

Henry Stewart talks

Language:

English

Subjects (All):

Interferon.

Physical Description:

1 videorecording (28 min., 00 sec.) : sound, color.

Other Title:

RNAi specificity

Place of Publication:

London : Henry Stewart Talks, 2016.

System Details:

video file

Summary:

Audio-visual presentation : siRNA design and use in mammalian cells ; Avoiding off-target effects ; Sense strand inactivation ; Evading interferon/stress response ; Smith-Waterman algorithm ; Human, mouse and rat Stealth(tm) Select ; Successful experimental design ; Appropriate RNAi controls.

Contents:

Printable Handouts

Navigable Slide Index

Introduction

Overview of topics

RNAi in mammalian cells

Definitions

Off-target effects detected by expression profiling

Advantages of chemically-modified siRNAs

RNAi design and activity

Sense strand inactivation

Stealth(tm) RNAi evades IFN stress response

Optimizing RNAi specificity by design

How can we increase specificity?

Smith-Waterman analysis

Stealth(tm) select RNAi

Stealth(tm) RNAi specificity by design

Stealth(tm) select activity

Smith-Waterman can reduce off-targets

General considerations

Successful experimental design

Thank you.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 12, 2024, from https://hstalks.com/bs/650/.