RTKs activate the ERK 1/2 MAPK cascade / Melanie H. Cobb.

Format:

Video

Author/Creator:

Cobb, Melanie H., author.

Series:

Henry Stewart talks

Language:

English

Subjects (All):

Cellular signal transduction.

Physical Description:

1 streaming video file (49 min., 15 sec.) : sound, color.

Place of Publication:

London : Henry Stewart Talks Ltd, 2007.

System Details:

video file

Contents:

Introduction

RTKs activate the ERK1/2 cascade

Ras mediates activation of the ERK 1/2 cascade

MAP kinase cascade paradigm

Multiple MAP kinase pathways in mammalian cells

Properties of MAPK cascades

MAPK cascade

Activation of Raf-1

MEK1

Structure of ERK2

Effect of phosphorylation on ERK2

Prop. of MAPK cascades-specificity generation

ERK1/2 assays

Enzymatic specificity of MEKs

Function of MEK1

Importance of proline-rich insert in MEK1

Activation of ERK1/2 in cells requires MEK1 insert

MEK1 binds stably to ERK2

Recognition sites on ERK2

MEK1 - function of ERK docking site in cells

Docking interactions required for ERK2 phosphor.

ERK2 interactions

Mutations that decrease ERK2 activation by MEK1

Behavior of ERK2 MEK-binding mutants

ERK1/2 scaffolding proteins direct distinct outputs

KSR scaffolds the ERK cascade

MEKK1 scaffolds MAPK cascades (1)

MEKK1 scaffolds MAPK cascades (2)

Threshold and ultrasensitivity in ERK1/2 pathway

Analysis of ERK2 from cells

Signal transduc. mechanisms-coincidence/synergy

Properties of MAPK cascades - signal modulation

Feedback phosphorylation of SOS

Signal transduction mechanisms-feedback

Properties of MAPK cascades-localization

Ligand-dependent diff. in ERK1/2 localization

ERK2 function depends on localization

Classical nuclear import and export

How does ERK2 enter and exit the nucleus?

pERK2 dimer interface

PEA-15 - protein enriched in astrocytes of 15 kDa

PEA-15 reduces nuclear import of GFP-ERK2

PEA-15 inhibits binding of ERK2 to nucleoporins

Localization of ERK1/2 influences functions

Properties of MAPK cascades - functions

ERK1/2 are required for cell migration

ERK1/2 required for insulin gene transcription (1)

ERK1/2 required for insulin gene transcription (2)

Properties of MAPK cascades - multifunctionality

Signal transduction mechanisms - modulation

ERK1/2 sensitivity to cAMP depends on act. ligand

ERK1/2 and ERK5 synergize to transform cells

Differential action of cAMP on ERK1/2 and ERK5

Acknowledgements.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 13, 2024, from https://hstalks.com/bs/462/.