Visualizing and manipulating phosphoinositide and phospholipid signaling / Glenn D. Prestwich.

Format:

• Video

Author/Creator:

• Prestwich, Glenn D., author.

Language:

English

Subjects (All):

• Phospholipids.
• Cellular signal transduction.

Physical Description:

1 online resource (1 streaming video file (55 min.)) : sound, color

Place of Publication:

London : Henry Stewart Talks Ltd, 2022.

System Details:

video file

Contents:

• Introduction
• Lipids regulate cellular communication
• The phosphoinositide polyphosphates
• Signaling as viewed from Salt Lake City
• PI localization in membrane trafficking pathways
• Synthetic phosphoinositide probes
• Intracellular delivery of phosphoinositides (1)
• Intracellular delivery of phosphoinositides (2)
• Intracellular delivery of PI into NIH 3T3 fibroblasts
• Reconstruction of PI(3,4)P2-NBD and histone-TMR
• Ins(1,4,5)P3 and PtdIns(4,5)P2 stimulate Ca fluxes
• Calcium oscillations initiated by shuttled PI(4,5)P3
• Exogenous phosphoinositides elicit cell responses
• Neutrophils polarize and follow chemoattractants
• PtdIns(3,4,5)P3 induces polarization and migration
• PI 3-K and RhoGTPase required for polarization
• PI(4)P rescues recruitment of AP-1 to golgi
• PI(3,4,5)P3 causes GLUT4 incorporation
• Metabolically-stabilized phosphoinositides
• Phosphoinositides are remodeled by enzymes
• Phospholipase C-resistant PI(4,5)P2 analogs
• Synthesis of stabilized PtdIns(4,5)P2 analogs
• PLC-resistant PtdIns(4,5)P2 analogs
• PLC-resistant PtdIns(4,5)P2 - no Ca2+ release
• Phosphatase-resistant PtdIns(3)P analogs
• PI(3)P analogs bind to FYVE and PX domains
• Are ms-PI(3)Ps substrates for PIKfyve?
• Ms-PI(3)Ps are phosphorylated by PIKfyve
• Phosphatase-resistant PtdIns(3,4,5)P3 analogs
• New chemistries for PIP tethering
• Synthetic two-headed lipids: PE and PIP heads
• SPR: Grp1 binds biotinylated Hy-PIP4
• Hy-PIPn-TMR self-shuttles to plasma membrane
• Head group spin-labeled Hy-PI(3)P
• Fluorescent Hy-PIPsTM in plasma membrane
• Hy-PIPsTM: better than acyl-modified PIPs
• PolyPIPosomesTM: polymerized PIPn-liposomes
• Comparing binding kinetics by SPR
• Tethers compared: global SPR analysis
• Relative affinities from SPR
• The PHD finger of RAG2 modulates V(D)J
• PolyPIPosomesTM: advantages
• Fishing with tethered IPs and PIPs
• Functional lipidomics: the next frontier
• Different baits catch different fish
• ING2 first isolated as a PI(3,4,5)P3 binding protein
• ING2 PHD binds PI(5)P selectively
• Nuclear ING2 lipid-Zn finger complex
• Co-localization of ING2 PHD and TR-PI(5)P
• Mining for signaling by functional proteomics
• Mass spec identifies protein complexes
• Functional proteolipidome
• Anti-PIP antibodies
• Anti-PI(3,4,5)P3 immunogen: a tool to detect PIP4
• PIP3 and PIP2 in PDGF-stimulated 3T3 fibroblasts
• PI(4,5)P2 co-localizes with nuclear markers
• Insulin and leptin activate PI3-K
• Anti-PI(4,5)P2 co-localizes with PLCd PH domain
• Defective signaling leads to excess PIP4
• Lipid signaling oncogenes in ovarian cancer
• Immunodetection of PI(3,4,5)P3 in tissues
• Visualizing PLA2 and PLD activity
• Fluorogenic substrates to measure PLA activity
• DBPC and BBPC fluorogenic cleavage by sPLA3
• cPLA2 activity in living MDCK-D1 cells
• Detection of iPLA2 activity in cancer cells
• Oxo-M stimulates fatty acid release via cPLA3
• Enzyme-assisted fluorogenic substrates synthesis
• Head group selectivity of PLA hydrolysis
• Fluorogenic assay for PLD activity
• Enzyme-assisted synthesis of PLD substrates
• Dimethyl analog with Strep. chromofuscus PLD
• Summary.

Notes:

• Description based on publisher supplied metadata and other sources.
• Retrieved April 12, 2024, from https://hstalks.com/bs/940/.