Titin I27 : a protein with a complex folding landscape / Jane Clarke.

Format:

Video

Author/Creator:

Clarke, Jane, author.

Language:

English

Subjects (All):

Proteins--Conformation.

Proteins.

Physical Description:

1 online resource (1 streaming video file (38 min.)) : sound, color

Other Title:

Titin I27

Place of Publication:

London : Henry Stewart Talks Ltd, 2007.

System Details:

video file

Contents:

Introduction

Titin I27 - general description

Folding - unfolding plot for Titin I27

Environmental conditions change folding pathway

TI27 mutants - unfolding pathways

Parallel folding pathways

How can we analyze these data?

High and low-stress unfolding pathway

Temperature can change the unfolding pathway

Mutations can change the folding pathway

Titin - a protein experiencing mechanical stress

Titin - effects of force

How does Titin resist unfolding under stress?

Addition of force lowers the unfolding energy barrier

Using AFM to investigate protein unfolding

First experiments - using whole proteins

The ideal protein - 8 repeats of I27

The protein - 8 repeats of I27 - unfolding

AFM experiments let comparison with simulations

Simulations - first step - forming an intermediate

Humps appear on the plot of unfolding

Mutation in the A-strand does not affect unfolding

A-strand lacking intermediate is stable

TI I27 has an intermediate on the unfolding pathway

Protein engineering for analysis of unfolding

Rationale

Different mutanst affect the folding force differently

Titin forced unfolding pathway

TS under force vs. physiological conditions

What can alter the protein unfolding landscape

Single molecular experiments let us see rare events

How do multi-domain proteins avoid aggregation

Tandem proteins aggregate faster

Strategies of aggregation avoidance

Titin domains behave independently

Strategy 2 - weak modules next to strong modules

Strategy 3 - select for certain residues

Removing the prolines speeds up aggregation

Strategy 4 - diversify the sequence of domains (1)

Strategy 4 - diversify the sequence of domains (2)

Evolutionary pressure to diversify sequences

How similar are Titin domains?

Conclusion.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 13, 2024, from https://hstalks.com/bs/432/.