TRAIL-induced apoptosis : from analysis of the death-inducing signalling complex (DISC) to clinical applicability / by Dr. Henning Walczak.

Format:

Video

Author/Creator:

Walczak, Henning, author.

Language:

English

Subjects (All):

Apoptosis--Research.

Apoptosis.

Ligand binding (Biochemistry).

Physical Description:

1 videorecording (27 min., 17 sec.) : sound, color

Other Title:

TRAIL-induced apoptosis

Place of Publication:

London : Henry Stewart Talks Ltd, 2007.

System Details:

video file

Summary:

Audio-visual presentation : poptosis induction by the TNF-related apoptosis-inducing ligand (TRAIL) ; Apoptosis-inducing TNF super-family ligands and receptors ; Formation of the deathinducing signalling complex (DISC) ; Caspase-8 versus caspase-10 ; Synergy of TRAIL with chemotherapeutics ; Tumour specificity of TRAIL-induced apoptosis ; Regulation of sensitivity versus resistance towards TRAIL-induced apoptosis.

Contents:

Introduction

Apoptosis induction by TRAIL

TRAIL: a member of the TNF superfamily

TNF-related apoptosis-inducing ligand (TRAIL)

TRAIL-R belong to the TNF-R superfamily

The death receptors

The TRAIL system

TRAIL DISC formation

c-FLIPS/c-FLIPL inhibit TRAIL-mediated apoptosis

Caspase 10

Caspase-10 is recruited to CD95 and TRAIL

Caspase-10 cannot substitute caspase-8

Caspase-10 protein expression is lost in tumors

Isoforms of caspase-10 and caspase-8

No differential cleavage for RIP and TRAF-1

Caspase-3 is cleaved by caspase-8 and -10

Caspase-8 and -10 generates different proteins

Summary and conclusion (caspase-8 versus -10)

Death receptors

The TRAIL system: can we kill tumor cells?

LZ-TRAIL induces apoptosis in tumor cell lines

Effect of TRAIL on normal human cells in vitro

TRAIL treatment of a subcutaneous tumor

Efficacy of TRAIL treatment in tumors

Inhibition of etoposide-induced apoptosis by Bcl-x

Double hit on tumor cells

Safety of TRAIL treatment: alone and in synergy

TRAIL versus CD95L in vivo

Sensitivity of PHH to TRAIL and to CD95L

Sensitivity and resistancy of PHH to TRAIL

HepG2 sensitivity to various forms of TRAIL

PHH are sensitive to one form of TRAIL only

Sensitisation of PHH by chemotherapeutics

Summary and conclusion (TRAIL on PHH)

What determines TRAIL sensitivity vs. resistance?

Sensitivity of various Hep cells to TRAIL

Proteasome inhibitors sensitise HC for TRAIL

Proteasome inhibition: TRAIL-induced apopotosis

Proteasome inhibition results in NF-kB inhibition

Proteasome inhibitors: upregulation of TRAIL-R1,2

TRAIL upregulation unnecessary for sensitisation

Proteasome inhibition enhances TRAIL formation

TRAIL sensitisation at the DISC level

Crosstalk between the two death pathways

Division of apoptosis regulation (DO40)

Thank you.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 12, 2024, from https://hstalks.com/bs/285/.