Plasmid rolling-circle (RC) replication / Saleem A. Khan.

Format:

Video

Author/Creator:

Khan, Saleem A., author.

Language:

English

Subjects (All):

Plasmids.

Physical Description:

1 videorecording (30 min., 18 sec.)

Other Title:

Plasmid rolling-circle

Place of Publication:

London : Henry Stewart Talks Ltd, 2008.

System Details:

video file

Summary:

Audio-visual presentation: Rolling circle replicating (RCR) plasmids -- Functional organization of rolling-circle replicating plasmids -- Model for plasmid rolling-circle replication -- Initiation and termination of replication in plasmids carrying two pT181 dsos -- DNA sequence and structure of the pT181 origin -- Initiator proteins of RCR plasmids -- Comparison of the replication origins of the plasmids of pT181 family -- Rep-DNA specificity -- Role of individual monomers of the Rep protein -- Purification of RepC heterodimers -- PcrA helicase -- Role of PcrA helicase in plasmid RC replication -- Conversion of ss to ds DNA -- Single strand origins of replication -- Lagging-strand replication.

Contents:

Introduction Rolling-circle replicating (RCR) plasmids Characteristicts of RCR plasmids Functional organization of RCR plasmids A model for plasmid rolling-circle replication Replication in plasmids carrying two pT181 dsos DNA sequence and structure of the pT181 origin Initiator proteins of RCR plasmids (1) Initiator proteins of RCR plasmids (2) DNA nicking-closing activity of RepC Domain structure of RepC initiator - pT181 plasmid Conservation of the active tyrosine residue DNA binding domains of pT181 family initiators Replication origin of the pT181 family plasmids Rep-DNA specificity Role of individual monomers of the Rep protein Purification of RepC heterodimers SDS-PAGE analysis of purified RepC heterodimers Division of labor between the two monomers PcrA helicase Role of the PcrA helicase in RC replication Conversion of ss to ds DNA General features of single-strand origins (SSOs) Types of single strand origins of replication SsoA origins of RC plasmids Synthesis of primer RNAs from plasmid ssoA Binding of staphylococal RNAP to pLS1/pE194 Binding of RNA polymerase to the ssoU ssoA sequences of pLS1 and pE194 plasmids pUB110/pMV158 ssoU Model of lagging-strand synthesis from ssoA Lagging strand replication - conclusions Important unresolved issues.

Notes:

Description based on: online resource; title from PDF information screen (Henry Stewart Talks Business & Management Collection, viewed April 16, 2024).

Retrieved April 16, 2024, from https://hstalks.com/bs/703/.