Peroxynitrite biochemistry oxidation and nitration reactions / Rafael Radi.

Format:

Video

Author/Creator:

Radi, Rafael, author.

Language:

English

Subjects (All):

Oxidative stress.

Physical Description:

1 streaming video file (42 min.) : digital, mono, SWF file, sound, color

Place of Publication:

London : Henry Stewart Talks Ltd, 2018.

System Details:

video file

Summary:

Audio-visual presentation : Peroxynitrite formation ; Peroxynitrite reaction and decomposition pathways: reaction of ONOOH with thiols ; Cysteine oxidation by peroxynitrite ; Reactivity of thiols with peroxynitrite ; Reaction of peroxynitrite with transition metal centers ; Peroxynitrite-dependent metal-catalyzed nitration ; Reaction of ONOO- with carbon dioxide ; Peroxynitrite reaction with amino acids ; Formation of secondary radical species ; Radical pathways of tyrosine nitration ; Mitochondrial nitration ; Cytochrome C and tyrosine nitration ; Mechanisms of cell death induced by peroxynitrite.

Contents:

Introduction

Peroxynitrite oxidation of sulfhydryls

SOD and peroxynitrite formation

Peroxynitrite and decomposition pathways

Direct reactions vs. the hydroxyl radical pathway

Kinetics of peroxynitrite reaction with biotargets

Reaction of ONOOH with thiols

Stopped-flow studies of cysteine oxidation

Second order rate constant determination

pH profile of cysteine oxidation by peroxynitrite

Reactivity of thiols with peroxynitrite

Thiol pKSH and reactivity with peroxynitrite

Kinetics of TXNPx reaction with peroxynitrite

A series of values of 2nd order rate constants

Peroxynitrite reactions with thiols

Peroxynitrite and metal centers reaction

Reaction of metalloporphyrins with peroxynitrite

Nitration and inactivation of manganese SOD

Peroxynitrite-dependent metal-catalyzed nitration

Human hemoglobin reaction with peroxynitrite

Reaction of ONOO- with carbon dioxide

Peroxynitrite-induced chemiluminescence

Peroxynitrite reacts fast with carbon dioxide

Direct EPR detection of carbonate radical

Carbonate radical from ONOO-/CO2 reaction

Rate constants of reaction with target molecules

Peroxynitrite reaction with aminoacids kinetics

Apparent hydroxyl radical production by ONOO-

Formation of secondary radical species

Lipid peroxidation and nitration

Radical pathways of tyrosine nitration

Protein 3-nitrotyrosine immunostaining

Protein tyrosine nitration

Formation of ONOO- derived species

Mitochondrial nitration

Cytochrome c and tyrosine nitration

Cytochrome c heme pocket

Anti-nitrotyrosine binding to cytochrome c

MS analysis of cytochrome c

Preferentially nitrated tyrosine amino acids

ONOO- treated cytochrome c: HPLC and MS

Hydrophobic analogs for tyrosine nitration study

New aspects of protein tyrosine nitration

Tyrosine oxidation and nitration: the steps

Overview

Peroxynitrite-induced mechanisms of cell death

Updated review on peroxynitrite

Conclusions

References

Acknowledgements.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 16, 2024, from https://hstalks.com/bs/377/.