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Ubiquitin dependent degradation of proteins modified by oxidation, deamidation and glycation : role in vision / Allen Taylor.

Henry Stewart Biomedical & Life Sciences Collection Available online

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Format:
Video
Author/Creator:
Taylor, Allen, author.
Language:
English
Subjects (All):
Amyloidosis.
Free radicals (Chemistry).
Physical Description:
1 streaming video file (52 min.) : digital, mono, SWF file, sound, color
Other Title:
Ubiquitin dependent degradation of proteins modified by oxidation, deamidation and glycation
Place of Publication:
London : Henry Stewart Talks Ltd, 2017.
System Details:
video file
Summary:
Audio-visual presentation : Talk to be added shortly Description of cataract and AMD as the ultimate protein misfolding diseases ; Lens development: a model for aging research ; Photo-oxidative stress to the retina ; Etiology ; Environmental factors ; Pathophysiology ; Protein quality control ; Ubiquitin pathway selectivity for oxidatively modified, glutathiolated, deamindated and glycated substrates ; Clinical relevance.
Contents:
Introduction
Talk outline
Normal eye illustration
Mouse lens cataract range
Normal clear lens vs. cataract lens
Lens brunescence and clarity vary independently
Lens development: a model for aging research
Protein precipitation disease
Cataract prevalence increases with age
The retina
The structure of the human retina
Photo-oxidative stress to the retina
The life cycle of the RPE
Progress of retinal degeneration
Distorted central vision due to AMD
View through AMD-damaged retina
AMD prevalence increases exponentially by age
AMD and cataract will increase within two decades
Loss of sight - second greatest fear of the elderly
Therapeutic opportunities
Etiology, environmental factors, pathophysiology
The eye deals with different kinds of stress
Smoking and AMD
Proteins are damaged upon oxidative stress
Oxidative damage to lens proteins
Formation of protein carbonyls
Oxidation reactions and sequela reaction products
Cataract, decreased P-SH and increased carbonyl
Glycoprotein vs. age in human lens
Lens protein distribution with age
Modifications to proteins/proteases in older tissues
Active proteases remove damaged proteins
Ubiquitin pathway selectivity for substrates
The ubiquitin-proteasome pathway (UPP)
Ubiquitin chain linkage and function
Oxidatively modified proteins in HLEC
Could we isolate ubiquitin conjugates?
K6W-Ub is conjugation competent
Selectivity of ubiquitination for oxidized proteins
Impairing the UPP by expression of K6W-Ub
Protease inactivation and damaged proteins
Lens clearity depends on a functional Ub pathway
Glutathiolation and gammaC-crystallin degradation
Glutathiolation enhances protein degradation rate
Proteolysis of GSH-modified gamma-C-crystallin
Degradation of glutathiolated gamma-C-crystallin
Glutathiolation and CAIII degradation
Minor CAIII structural alteration upon GSH
E1 and E2 are thiol enzymes
Thiolation of E1 following exposure to H2O2
Control of the UPP by GSSG:GSH
Oxidative inactivation of the proteasome
Protein deamidation progresses with age
Methylglyoxal reacts with Lys and Arg of proteins
Glycation/MGO decreases degradation
Clinical relevance
Simple sugars might cause cataract and AMD
High dietary glycemic index - risk for early ARM
Antioxidants can delay aging
What happens upon aging in the Ub pathway?
Summary.
Notes:
Description based on publisher supplied metadata and other sources.
Retrieved April 17, 2024, from https://hstalks.com/bs/393/.

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