Virulence and antibiotic resistance regulation in pathogens / Chuan He.

Format:

Video

Author/Creator:

He, Chuan, author.

Language:

English

Subjects (All):

Drug resistance in microorganisms.

Virulence (Microbiology).

Physical Description:

1 online resource (1 streaming video file (44 min.)) : sound, color

Place of Publication:

London : Henry Stewart Talks Ltd, 2011.

System Details:

video file

Contents:

Introduction

Threats from pathogens

Strategies to treat infections

Antibiotic resistance

E. coli MarR

Bacterial multidrug resistance regulators

MarR family transcriptional factors in S. aureus

Susceptibility of S. aureus mutants to NOR

Resistance to quinolone for S. aureus strains

Virulence test using the murine abscess model

Crystal structure of MgrA

Molecular mechanism for MgrA-based regulation

A unique Cys residue

Oxidation sensing?

Cysteine sulfenic acid formation in MgrA

Gel-shift assay to study MgrA-DNA interaction

Bio-relevance: plate sensitivity assays

Further in vivo results

MgrA is a redox switch in S. aureus

SarZ, an MgrA homologue in S. aureus

SarZ is also an oxidation sensor

SarZ plays a complementary role next to mgrA

Crystal structure of the reduced SarZ

Crystal structure of oxidized SarZ with Cys13-SOH

SarZ and mgrA are major ROS in S.aureus

How S. aureus senses host immune defense

High virulence/low virulence hypothesis

Virulence suppression strategies

PVS works like hydrogen peroxide

An epoxide as a mild and selective alkylator

Phenyl epoxide sulfonate (PES) activates MgrA

The in vivo effect of the epoxide

Developing small molecule modulators

FA-based HTS for non-covalent modulators

NSRB at Harvard

MgrA homologues in other pathogens

Two MgrA homologues in P. aeruginosa

OspR in P. aeruginosa

Cys24 in OspR is redox active

OspR plays a role in virulence

P. aeruginosa MexR

MexR involved in regulating resistance: hypothesis

MexR oxidation leads to its dissociation from DNA

Intermonomer disulfide bond formation in MexR

Computational analysis of oxidized MexR structure

Redox potential measurement of MexR

Experiments probing in vivo response

MexR senses peroxide stress

Crystal structure of the oxidized MexR

Conclusion.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 11, 2024, from https://hstalks.com/bs/1972/.