The kinetochore as a target for the development of mitosis specific anti-cancer drugs / Dr. Tim Yen - Fox Chase Cancer Center, Philadelphia, USA.

Format:

Video

Author/Creator:

Yen, Tim J., author.

Language:

English

Subjects (All):

Cancer--Chemotherapy.

Cancer.

Cell cycle.

Mitosis.

Physical Description:

1 videorecording (48 mins., 16 sec.) : sound, color

Place of Publication:

London, England : Henry Stewart Talks Ltd, 2009.

System Details:

video file

Contents:

Introduction

Talk topics

The kinetochore

Molecular composition of the human kinetochore

Kinetochore proteins assemble in a temporal order

CENP-F localization at different stages of G2

Defining temporal orders of assembly

FRAP studies and kinetochore

Turnover dynamics of kinetochore proteins

What is the kinetochore?

CENP-E: a kinesin like motor

CENP-E exhibits a dynamic localization pattern

CENP-E is essential for microtubule attachment

The HEC1/Ndc80 complex

Different phenotypes for various depletions

Three steps of the mitotic checkpoint pathway

Checkpoint proteins localize to kinetochores

The kinetochore and its relation to cancer

The mitotic checkpoint and cancer

Mitotic checkpoint genes are essential

Mitotic checkpoints and genome instability

The kinetochore as a mitosis specific target

Inhibitors of mitosis

Ansamycins are inhibitors of Hsp90

17-AAG blocks assembly of CENP-H and I

Histone deacetylase inhibitors

HDI delays human tumor cell lines in mitosis

HDI interferes protein assembly onto kinetochore

Not all kinetochore localized proteins are affected

The depletion is not a result of degradation

FTI's block farnesylation of proteins

FTI-2153 alters the localization of CENP-F

Effect of FTI on lung cancer cells

Inhibitors of kinetochore/spindle proteins

Kinase inhibitors-Aurora and Plk1

Polo kinases - Plk1-4

Pros and cons of targeting the mitotic checkpoint

Linking chromosome missegregation to cell death

The checkpoint and apoptosis pathways

Hela cells depleted of hNuf2 accumulate in mitosis

Timelapse videos of mitotically arrested cells

Hela cells blocked in mitosis undergo apoptosis

Patient response

Conclusions and future directions.

Notes:

Description based on: online resource; title from PDF information screen (Henry Stewart Talks Biomedical and Life Sciences Collection, viewed April 5, 2024).

Retrieved April 10, 2024, from https://hstalks.com/bs/1273/.