Innate recognition of viruses / Caetano Reis e. Sousa.

Format:

Video

Author/Creator:

Sousa, Caetano Reis e., author.

Language:

English

Subjects (All):

Natural immunity.

Physical Description:

1 streaming video file (46 min.) : digital, mono, SWF file, sound, color

Place of Publication:

London, England : Henry Stewart Talks Ltd, 2009.

System Details:

video file

Contents:

Introduction

T cell activation

Dendritic cells (DC)

Immune activation (1)

Pattern recognition

Bug recognition by Toll-like receptors in mammals

PRRs: coupling innate and adaptive immunity

Immune activation (2)

Viruses induce type I/III interferons

Induction of type I interferons is pattern recognition

Pattern recognition of viruses

IFN-alpha production and cytoplasmic dsRNA

IFN-alpha/beta induction by cytoplasmic dsRNA

Influenza virus

Influenza NS1 protein blocks IFN-a/b production

Cytoplasmic recognition of flu

Induction of IFN-a does not correlate with dsRNA

RIG-I mediated flu sensing

Transfection with flu vRNA is sufficient to elicit IFN

RIG-I binds directly to flu vRNA

RIG-I mediated sensing of flu genomes

Induction of type I/III interferons

Recognition is specific to vRNA

CIP abrogates the ability of flu vRNA to induce IFN

Conclusions (1)

5' PPP RNA binds to the CTD of RIG-I

Why RIG-I doesn't mediate responses to EMCV

What about self ssRNA?

The actual RIG-I agonist during virus infection

IFN-a/b induction by viruses - Cytosolic pathways

Mouse DC subsets

pDC make lots of IFN-a in response to wild type flu

Conclusions (2)

pDC responses to flu require TLR7

Endosomal recognition of viruses

Virus recognition pathways

TLR7 recognises poly U clusters

TLR7 recognises self mRNA

Conclusions (3)

What about apoptotic cells? (1)

Spleen DC subsets

Scavenging by CD8-alpha positive DC

What about apoptotic cells? (2)

CD8a+ DC do not express TLR7 but express TLR3

DC "decode" signs of infection within dying cells

Detecting virally-infected cells

Replication of some viruses generates dsRNA

DC activation by virally-infected cells

Virus-infected cells induce CTL responses

CTL priming after TLR3-mediated dsRNA sensing

Summary

Acknowledgements.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 8, 2024, from https://hstalks.com/bs/1305/.