Role of ER stress in cystic fibrosis airway inflammation / Carla Maria Pedrosa Ribeiro.

Format:

Video

Author/Creator:

Ribeiro, Carla Maria Pedrosa, author.

Language:

English

Subjects (All):

Lungs--Diseases, Obstructive.

Lungs.

Physical Description:

1 streaming video file (38 min.) : digital, mono, SWF file, sound, color

Place of Publication:

London : Henry Stewart Talks Ltd, 2012.

System Details:

video file

Contents:

Introduction

Cystic fibrosis

CF airways disease

Ca levels in CF human airway epithelia cultures

Ca mobilization in airway epithelia cells

Apical GPCR activation and Ca signaling

Apical ER/Ca store expansion

Summary of earlier studies

Modeling the CF phenotype

Mucosal SMM promotes ER/Ca store expansion

Apical ER/Ca store expansion- Cl- secretion

Apical ER/Ca store expansion-hyperinflammation

SMM affects inflammatory mediator production

SMM affects metabolism and protein synthesis

Hypothesis: causes of ER/Ca store expansion

ER unfolded proteins activate UPR pathways

IRE1 pathway

Airway epithelial adaptive response hypothesis

SMM induces XBP-1 mRNA splicing

SMM induces ER protein chaperone expression

Increased XBP-1s, BIP and PDI in human CF HBE

XBP-1 mRNA splicing coupled to IL-8 secretion

The role of XBP-1 in ER/Ca2+ store expansion (1)

Spliced XBP-1 promotes ER Ca store expansion

The role of XBP-1 in ER/Ca2+ store expansion (2)

DN-XBP-1 inhibit ER Ca store expansion

The role of spliced XBP-1 in IL-8 secretion

IRE1 in airway epithelial hyperinflammation

IRE1 isoforms: IRE1alpha and IRE1beta

16HBE14o- cells only express IRE1alpha

Studying ER Ca store expansion in vivo

XBP-1 splicing - ER expansion coupling

XBP-1 splicing is increased in inflamed airways

Future research directions

Summary

Acknowledgments.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 13, 2024, from https://hstalks.com/bs/2223/.