Reversing DNA methylation pharmacologically / Dr. Allen Yang.

Format:

Video

Author/Creator:

Yang, Dr. Allen, author.

Language:

English

Subjects (All):

DNA--Methylation--Research--Methodology.

DNA.

DNA--Methylation.

Physical Description:

1 videorecording (46 min., 16 sec.)

Place of Publication:

London : Henry Stewart Talks Ltd, 2010.

System Details:

video file

Contents:

Introduction

Overview

Targeting DNA methylation

Epigenetics in cancer

Hallmarks of cancer altered by methylation

Knudson's two-hit hypothesis

Secondary cancers from chemotherapy

DNA methylation, decitabine, and azacitidine

How DNA methyltransferase inhibitors work?

Muscle differentiation induced by 5-azacytidine

Maintenance of DNA methylation

Inhibition of methylation vs. cytotoxicity

Azacitidine vs. decitabine

Vidaza (azacitidine) and Dacogen (decitabine)

Hypomethylating agents and hydroxyurea

Hydroxyurea effect

Aphidicolin effect

Cytidine analogs

Clinical experience of azacitidine and decitabine

Induction of epigenetic changes by 5-azacytidine

Clinical results of low dose decitabine

Azacitidine survival study

Overall survival: azacitidine vs. CCR

DNMTi in solid tumors

Azacitidine in solid tumors

Identifying the target

How do you measure DNA methylation?

Surrogate for global DNA methylation

Decitabine treatment in CML patient

Decitabine: methylation and clinical response

Decitabine: methylation dose response

Phase 1 clinical study of azacitidine

Treatment schema

Results: LINE-1 DNA methylation changes

DNA repetitive elements serve as a surrogate

Summary of clinical translational data

Identifying specific gene targets

Targets of DNA methylation in cancer

Illumina analysis of APL, CML and GIST

CML and APL hypermethylated genes algorithms

ABL methylation is specific to CML patients

ID4 methylation and cancer progression

DNA methylation in CML

Genes involved in CML progression

Next generation epigenomic technology

Azacitidine treatment of MDS: methylation

The role of the Philadelphia chromosome

Next generation DNMTi

Degradation of 5-aza-nucleosides

What's next?

5-aza-2'-deoxycytidine and S110

Incorporation of azacytosine into a oligonucleotide

Capecitabine (Xeloda)

Reactivation of GFP is dependent upon CES1

Summary

Acknowledgements.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 12, 2024, from https://hstalks.com/bs/1748/.