Mitotic chromosome condensation / Prof. Andrew Belmont.

Format:

Video

Author/Creator:

Belmont, Andrew, author.

Series:

Henry Stewart talks

Language:

English

Subjects (All):

Cell cycle.

Physical Description:

1 videorecording (49 min., 55 sec.) : sound, color.

Place of Publication:

London : Henry Stewart Talks Ltd, [2009]

System Details:

video file

Contents:

Introduction

Outline

Perspective: million fold multiplier

Evolutionary and developmental comparisons

Phenomenology: stages of mitosis

Stages of mitosis (1)

Stages of mitosis (2)

Primary functions of mitosis

Chromosome length and chromosome segregation

Lower levels of chromatin organization

Two common observations

Early whole mount electron microscopy

Helical folding at highest level of compaction

What we thought we knew

Key advance

Micrographs of mitotic chromosomes

Metaphase chromatid cross section

The radial loop model

Interpretation

SARs and MARs

DNA sequences at the base of chromatin loops

Nonhistone proteins make up scaffold

Sc1 = topoisomerase 2

Condensin complex containing Sc2 (1)

Condensin complex containing Sc2 (2)

Condensin complex containing Sc2 (3)

Condensin complex containing Sc2 (4)

SMC family of proteins

Vindication of radial loop/scaffold model

Textbook model: condensation by condensins

Challenges to textbook model (1)

Challenges to textbook model (2)

200-300 nm subunit in metaphase chromosomes

Alternative model - Radial loop, Helical coil model

SMC2 gold staining doesn't support the coiling

Challenges to the textbook model (3)

Topo 2 dependant chromosome condensation

Chromosome condensation with topo 2 knockdown

Chromosome condensation with SMC2 knockdown

What we think we know

Phenotype of topo 2 and condensin knockdown

Condensins is required for chromosome stability

Two condensin complexes are present

Condensin 1 versus 2

What we'd like to know

Chromosome scaffold versus "glue" model

Possibilities for condensin knockdown results

Condensin activity and chromosome structure

Alternative model for chromosome condensation

Model

Structural studies and chromosome biochemistry

Chromosome structure: remaining questions (1)

Chromosome structure: remaining questions (2)

Recommended reviews.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 14, 2024, from https://hstalks.com/bs/1426/.