Proteins that bind methylated DNA / Pierre-Antoine Defossez.

Format:

Video

Author/Creator:

Defossez, Pierre-Antoine, author.

Language:

English

Subjects (All):

Proteins.

Proteins--Analysis.

Physical Description:

1 videorecording (30 min., 58 sec.) : sound, color

Place of Publication:

London : Henry Stewart Talks, 2011.

System Details:

video file

Contents:

Introduction

Talk overview

A few introductory notions

Reminder and graphic convention

Methylated DNA in 3D

The number of methylated CpGs per genome

The distribution of methylated CpGs in cells

DNA methylation and cancer

The identification of methyl-binding proteins (MBPs)

Why look for MBPs in the first place?

Biochemical identification of MBPs

Finding other MBPs by homology searches

Methyl binding proteins: Gel-retardation assay

Methyl binding proteins: Nuclear localization (1)

Methyl binding proteins: Nuclear localization (2)

Methyl binding proteins: Chromatin-IP

Human methyl-binding proteins list

What do the methyl-binding proteins do?

MBPs cause heterochromatinization

MeCP2, Rett syndrome, and neuronal transcription

Search for MeCP2 target genes in the mouse brain

MBD4, cancer, and mutagenesis

Spontaneous base deamination

UHRF1 is necessary for embryonic viability

UHRF1 and the maintenance of DNA methylation

Kaiso: a bifunctional transcription factor

Kaiso in colon cancer

Unresolved questions

Why are most MBPs non-essential?

Proteomics yield many new MBP candidates

New MBPs might be identified by genetic screens

The role of MBPs in active DNA demethylation?

Summary

Identification of methyl-binding proteins

Three families of methyl-binding proteins

General model

Acknowledgements

Reading suggestions

References.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 8, 2024, from https://hstalks.com/bs/1739/.