Phosphorylase and the origin of reversible protein phosphorylation / Prof. Edmond Fischer.

Format:

Video

Author/Creator:

Fischer, Prof. Edmond, author.

Language:

English

Subjects (All):

Phosphorylation.

Physical Description:

1 videorecording (44 min., 38 sec.)

Place of Publication:

London : Henry Stewart Talks Ltd, 2010.

System Details:

video file

Contents:

Introduction

55 years ago

Studying glycogen phosphorylase

Degradation of glycogen - adrenaline

Fight or flight

Insulin, glycogen synthase and glucagon

Louis Leloir

How this research started

Regulation of phosphorylase

Photograph of the Coris

Phosphorylase showed no requirement for AMP

Edwin Krebs - hesitation

Cori's preparation of muscle phosphorylase (1)

Purification of phosphorylase

Edwin Krebs - Cori's preparation

Ca2+ contamination is responsible for activation

Edwin Krebs - ashes experiment

What was phosphorylated?

Activation-inactivation cycle of phosphorylase

Edwin Krebs - JBC reviewers

Knowledge of phospho proteins in 1950

Ca2+ involvement in the crude extracts

Phosphorylase kinase is also phosphorylated

Sutherland

cAMP and hormonal control of glycogenolysis

Ca2+ and muscle contraction

Single fiber measurement method

Tension and phosphorylase kinase activity

Effect of the sarcoplasmic reticulum

The complete cycle of Ca2+

Ubiquitous protein phosphorylation

Phosphorylase was a good protein to work on

Reciprocal control

Control of glycogen synthesis and breakdown

Mechanism of activation of GSK-3

Not everybody believed in regulation by phos.

Looking back at the early days

The world - before and after

Thank you.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 12, 2024, from https://hstalks.com/bs/1878/.