Automated Ca2+ imaging of chemosensory neurones in C.elegans / Nikos Chronis.

Format:

Video

Author/Creator:

Chronis, Nikos, author.

Language:

English

Subjects (All):

Caenorhabditis elegans.

Physical Description:

1 streaming video file (28 min.) : color, sound

Place of Publication:

London : Henry Stewart Talks Ltd, 2012.

System Details:

video file

Contents:

Introduction

What C. elegans are

Neuronal networks - from stimulation to behavior

Monitoring neuronal activity - calcium imaging

In vivo Ca imaging of chemosensory neurons

Automating In vivo Ca imaging

An automated calcium imaging platform

In vivo Ca imaging using a microfluidic chip

Processing single worms in the microfluidic chip

Fabrication of the microfluidic chip

The automated, in vivo calcium imaging platform

Automated single worm processing on-chip

Automated calcium imaging on-chip

A typical Ca response (chemosensory neuron)

Automated calcium imaging - possibilities

Effects of age on the function of sensory neurons

In vivo calcium imaging - parameters

Age effect on ASH neuronal response

Peak and slope of the ON response

Initial intracellular Ca level may be age-dependent

Calcium pathway in ASH neuron

Using in vivo imaging for drug screening

Approach - drug-treated vs untreated worms

Mianserin - yes, Resveratrol - no

Summary

Behind the scene.

Notes:

Description based on publisher supplied metadata and other sources.

Retrieved April 12, 2024, from https://hstalks.com/bs/2272/.