Pocket guide to mycological diagnosis / [edited by] Rossana de Aguiar Cordeiro.

Format:

Book

Contributor:

De Aguiar Cordeiro, Rossana, editor.

Series:

Pocket guides to biomedical sciences.

Pocket guides to biomedical sciences

Language:

English

Subjects (All):

Medical mycology.

Mycoses--Diagnosis.

Mycoses.

Physical Description:

1 online resource (173 pages).

Edition:

1st ed.

Place of Publication:

Boca Raton : CRC Press, [2020]

Summary:

"The Pocket Guide to Mycological Diagnosis aims to provide useful and concise information for microbiologists and professionals interested in the diagnosis of the most relevant fungal species of medical importance. This book will allow mycologists to face the emergoing challenges of routine diagnosis"-- Provided by publisher.

Contents:

Cover

Half Title

Series Page

Title Page

Copyright Page

Contents

Preface

Editor

Contributors

List of Abbreviations

1. Mycological Diagnosis: General Principles

1.1 Introduction

1.2 Collection of clinical specimens

1.3 Mycological processing (conventional tests)

1.3.1 Direct microscopic examination

1.3.2 Culture

1.4 Identification

1.4.1 Standard culture-based methods: Phenotypical analysis

1.4.2 Culture-independent methods

1.4.2.1 Molecular diagnosis

1.4.2.2 Immunological diagnosis

1.4.2.3 Matrix-assisted laser desorption ionization-time of flight mass spectrometry

1.5 Conclusions

Bibliography

2. Antifungal Drugs and Susceptibility Testing of Fungi

2.1 Antifungal drugs

2.1.1 Cell membrane activity

2.1.1.1 Formation of pores on cell membrane

2.1.1.2 Inhibition of ergosterol synthesis

2.1.2 Intracellular activity

2.1.2.1 Griseofulvin

2.1.2.2 Pyrimidines

2.1.2.3 Pyridine derivatives

2.1.3 Cell wall activity

2.1.3.1 Echinocandins

2.2 Antifungal Susceptibility Testing

2.2.1 Methods for antifungal susceptibility testing

2.2.2 Minimum inhibitory concentration interpretation: Clinical breakpoints vs. epidemiological cutoff values

2.2.3 Automation in antifungal susceptibility testing

3. Candida spp.

3.1 General aspects

3.2 Laboratory diagnosis

3.2.1 Microscopy

3.2.2 Culture

3.3 Identification methods

3.3.1 Conventional

3.3.2 Semiautomatic or automatic platforms

3.3.3 Molecular identification

3.4 Therapeutic options

References

4. Cryptococcus

4.1 Introduction

4.2 Clinical diagnosis

4.3 Laboratory diagnosis

4.3.1 Direct exam

4.3.2 Capsular antigen detection

4.3.3 Isolation and identification of C. neoformans and C. gattii species

4.3.4 Histopathology

4.4 Therapy.

4.5 Prevention

5. Trichosporon

5.1 Introduction

5.2 Specimen selection, collection, and transport

5.3 Specimen processing in the mycology laboratory

5.4 Species identification

5.5 Rapid diagnostic tests

5.6 Serology

6. Malassezia

6.1 Introduction

6.2 Pathophysiology of superficial infections

6.3 Clinical manifestations

6.4 Laboratory diagnosis

6.4.1 Collection of clinical samples

6.4.2 Microscopy

6.4.3 Culture

6.4.4 Identification

6.5 Conclusions

7. Rhodotorula spp.

7.1 Introduction

7.2 Predisposing factors and clinical manifestations

7.3 Laboratorial diagnosis

7.3.1 Mycological diagnosis

7.3.1.1 Microscopic and culture findings

7.3.2 Identification tools

8. Dermatophytes

8.1 Historical aspects

8.2 Epidemiology

8.3 Clinical manifestations

8.4 Laboratory diagnosis

8.4.1 Growth on special culture media

8.4.2 Slide culture

8.4.3 Urease production

8.4.4 In vitro hair perforation test

8.4.5 Vitamin and nutritional tests

8.5 DNA-based molecular identification

8.6 Identification based on protein profile (matrix-assisted laser desorption/ionization-time of flight)

8.7 Main species of dermatophytes

8.7.1 The Microsporum genus

8.7.1.1 Microsporum canis

8.7.1.2 Microsporum ferrugineum

8.7.2 The Trichophyton genus

8.7.2.1 Trichophyton rubrum

8.7.2.2 Trichophyton tonsurans

8.7.2.3 Trichophyton interdigitale

8.7.2.4 Trichophyton verrucosum

8.7.2.5 Trichophyton violaceum

8.8 Final considerations

9. Aspergillus spp.

9.1 Introduction

9.2 Types and symptoms of aspergillosis

9.2.1 Invasive aspergillosis

9.2.2 Aspergilloma

9.2.3 Chronic fibrosing pulmonary aspergillosis

9.2.4 Allergic aspergillosis

9.2.5 Cutaneous aspergillosis.

9.3 Aspergillosis risk factors and prevention

9.4 Approach to diagnosis

9.5 Resistance in Aspergillus spp.

10. Mucorales

10.1 Introduction (epidemiology and common mucormycosis agents)

10.2 Predisposing factors and pathogenesis

10.3 Clinical features

10.4 Diagnosis

10.5 Therapy

11. Sporothrix spp.

11.1 Introduction

11.2 Virulence of Sporothrix species

11.3 Pathogenesis and clinical features

11.4 Sample collection

11.5 Direct examination: Mycology and histopathology

11.6 Culture of Sporothrix spp.

11.7 Molecular identification of Sporothrix species

11.8 Developments in the serology of sporotrichosis

11.9 Treatment of sporotrichosis and susceptibility to antifungals

Acknowledgments

12. Histoplasma capsulatum

12.1 Introduction

12.2 Collection, transport, and processing of clinical samples

12.3 Direct examination and culture

12.4 Cytopathology and histopathology

12.5 Immunodiagnostic tests

12.5.1 Antibody detection

12.5.2 Antigen detection

12.6 Molecular diagnostics

12.7 Summary and conclusions

13. Paracoccidioides Complex

13.1 Introduction

13.2 Mycological diagnosis of paracoccidioidomycosis

13.3 Collection, transport, and processing of clinical specimens

13.4 Direct examination

13.5 Histopathological examination

13.6 Cultural examination

13.7 Serological diagnosis

13.8 Molecular diagnosis

14. Coccidioides

14.1 Introduction: General aspects

14.2 Collection and transport of clinical samples for mycological diagnosis

14.3 Laboratory processing

14.3.1 Direct examination

14.3.2 Culture

14.4 Histopathological findings

14.5 Molecular diagnosis

14.6 Serological tests

14.7 Conclusion

15. Pneumocystis jirovecii.

15.1 Introduction

15.2 Collection, transport, and processing of samples

15.3 Microscopic examination and culture

15.4 Immunological test for P. jirovecii antibody detection

15.5 Serological biomarkers: (1-3)-ß-D-glucan, lactate dehydrogenase, Krebs von den Lungen-6, and S-adenosylmethionine

15.6 Molecular diagnosis of Pneumocystis jirovecii pneumonia

15.6.1 Conventional polymerase chain reaction

15.6.2 Nested polymerase chain reaction

15.6.3 Real-time polymerase chain reaction (qualitative polymerase chain reaction)

15.7 Combination testing

15.8 Summary and conclusions

Index.

Notes:

Description based on print version record.

ISBN:

1-351-67766-7

1-351-67767-5

1-315-16562-7

9781315165622

OCLC:

1114284967