Phase Separation in Living Cells : Benefits and Risks / edited by Riki Kurokawa.

Format:

Book

Contributor:

Kurokawa, Riki.

Series:

Biomedical and Life Sciences Series

Language:

English

Subjects (All):

Molecular biology.

Biophysics.

Medicine--Research.

Medicine.

Biology--Research.

Biology.

Cytology.

Biochemistry.

Non-coding RNA.

Molecular Biology.

Biomedical Research.

Cell Biology.

Non-coding RNAs.

Local Subjects:

Molecular Biology.

Biophysics.

Biomedical Research.

Cell Biology.

Biochemistry.

Non-coding RNAs.

Physical Description:

1 online resource (309 pages) : illustrations (black and white, and color).

Edition:

1st ed. 2023.

Place of Publication:

Singapore : Springer Nature Singapore : Imprint: Springer, 2023.

Summary:

This book presents the latest cutting-edge research on phase separation. It discusses the benefits and risks of phase separation for living cells from the perspectives of physics, chemistry, biology, and medicine. Phase separation is a physico-chemical process that induces a single solution of solvent and intrinsically disordered proteins (IDPs) to separate into two phases, one phase containing only solvent and the other containing IDPs in the solvent. A key molecule in phase separation is the intrinsically disordered region (IDR) proteins (IDPs), mostly comprised of RNA-binding proteins (RBPs). One of the major roles of phase separation is to generate condensates, membrane-less organelles including stress granule, Cajal body, and nucleolus. Biological actions of phase separation in various aspects of science have received increasing attention in recent years. The book consists of four parts; Part I on physics and chemistry includes topics on structural biology of RBP FUS/TLS and chaperone for phase separation, computational approach of phase separation, and chemistry of G-quadruplex of DNA and RNA. Part II on molecular biology presents molecular mechanism of IDR sequence phase separation and potential cellular function of these labile fibers, formation of membrane-less organelles, and roles of noncoding RNAs in phase separation. Part III on biology covers topics from nuclear pore complex, developmental biology regarding force-dependent remodeling, and neurobiology and higher order brain functions. Finally, Part IV on medicine presents condensates and cancer therapy, pathogenesis of neurodegenerative diseases based on IDPs, and responses of microglia to amyloid. The last chapter highlights the latest topic on how the SARS-Cov-2 virus takes advantage of phase separation for its infections. This book brings together the studies of phase separation in each area in a single volume. The comprehensive approach provides new insights to the research and will benefit the readers by responding to the emerging needs for understanding phase separation.

Contents:

Chapter 1. FUS aggregation by shear stress on pipetting and its suppression by non-coding RNA

Chapter 2. Basics and recent advances in computational and theoretical methods for understanding the liquid-liquid phase separation

Chapter 3.Promotion of liquid-liquid phase separation by G-quadruplex DNA and RNA

Chapter 4. Phase regulation by chaperons

Chapter 5. Regulation of aggregation of intrinsically disordered protein through phase separation: Risk management of phase separation

Chapter 6. Molecular mechanisms of phase transition/separation of protein low-complexity sequences

Chapter 7. Winding and Tangling. An Initial Phase of Membrane-less Organelle Formation (from a viewpoint of Cajal Bodies)

Chapter 8. Formation and function of phase separated nuclear bodies directed by architectural noncoding RNA

Chapter 9. Force-dependent remodeling of cell-to-cell adhesion through the regulation of phase separation

Chapter 10. Neuronal RNA granules: Phase separation, dynamics, and higher brain functions

Chapter 11. Liquid-liquid phase separation in structure and function of nuclear pore complex

Chapter 12. Phase Separation Orchestrates Cancer Signaling: Biomolecular condensates as a promising target for cancer therapy

Chapter 13. Regulatory interaction of intrinsically disordered regions of pathogenic proteins in neurodegenerative diseases

Chapter 14. Functional properties of phase separation and intranuclear complex of FUS in the pathogenesis of ALS/FTLD

Chapter 15. Functional responses of microglia to amyloid plaques

Chapter 16. Emerging role of phage separation in COVID-19.

Notes:

Description based on publisher supplied metadata and other sources.

Other Format:

Print version: Kurokawa, Riki Phase Separation in Living Cells

ISBN:

981-9948-86-X

OCLC:

1404264660