Protective Signalling Mechanisms in the Lung Induced by Open-Lung Ventilation Strategies.

Format:

Book

Author/Creator:

Fuest, Sven.

Language:

English

Subjects (All):

Respiratory distress syndrome.

Ventilation.

Physical Description:

1 online resource (75 pages)

Edition:

1st ed.

Place of Publication:

Cologne : Lehmanns Fachbuchhandlung GmbH, Abt. Verlag, 2014.

Summary:

This medical dissertation explores protective signaling mechanisms in the lungs during open-lung ventilation strategies. It provides a detailed analysis of lung architecture and physiology, including the roles of alveoli and cell types such as pneumocytes and fibroblasts. The study focuses on Acute Respiratory Distress Syndrome (ARDS), its pathology, phases, and implications for lung function. Utilizing animal models and laboratory techniques, the research investigates cellular responses to mechanical ventilation and aims to identify strategies that minimize lung injury. The work is intended for medical professionals and researchers specializing in pulmonology, critical care, and respiratory therapy. Generated by AI.

Contents:

Intro

Titel

Impressum

CONTENTS

1. INTRODUCTION

1.1. Architecture of the lung

1.2. Physiology of the lung

1.3. Acute respiratory distress syndrome

1.3.1. Pathophysiology

1.3.2. Animal models

1.4. Ventilator-induced lung injury

1.4.1. Pathophysiology

1.4.2. Lung protective ventilation

1.5. Intracellular mechanisms of converting mechanical stimuli

1.5.1. Responses to mechanical forces

1.5.1.1. Cell death

1.5.1.2. Pro-apoptotic pathways

1.5.1.3. Anti-apoptotic pathways

1.5.1.4. Mitogen-activated protein kinase pathways

1.6. Aim of the study

2. MATERIALS AND METHODS

2.1. Materials

2.1.1. Equipment

2.1.2. Reagents

2.2. Methods

2.2.1. Animal models

2.2.2. Protein isolation from lungs

2.2.3. Determination of protein concentration

2.2.4. SDS polyacrylamide gel electrophoresis

2.2.5. Protein blotting

2.2.6. Protein visualisation

2.2.7. RNA isolation

2.2.8. Measuring RNA concentration

2.2.9. cDNA synthesis

2.2.10. Real-time polymerase chain reaction

2.2.11. Cryosections - immunohistochemistry

2.2.12. Statistical analysis

3. RESULTS

3.1. Protein expression analysis

3.1.1. The mitogen-activated protein kinases

3.1.2. PI3K-Akt pathway

3.1.3. Markers of cell death

3.2. Gene expression analysis

3.3. Immunohistochemistry

4. DISCUSSION

5. ABSTRACT

6. ZUSAMMENFASSUNG

7. ABBREVIATIONS

8. REFERENCES

9. ERKLÄRUNG ZUR DISSERTATION

10. DANKSAGUNG.

Notes:

Description based on publisher supplied metadata and other sources.

Part of the metadata in this record was created by AI, based on the text of the resource.

ISBN:

9783865416162

3865416160

OCLC:

1482262307