Biophysical techniques / Iain D. Campbell.

Format:

Book

Author/Creator:

Campbell, Iain D., author.

Series:

Science trove.

Science trove

Language:

English

Subjects (All):

Biophysics.

Physical Description:

1 online resource (364 p.)

Place of Publication:

Oxford : Oxford University Press, 2023.

Language Note:

English

Summary:

'Biophysical Techniques' explains the basics of the various biophysical methods available so students can understand the principles behind the different methods used, and begin to appreciate which tools can be used to probe different biological questions, and the pros and cons of each.

Contents:

Cover; Contents; Some frequently used abbreviations; 1 Introduction; What are "biophysical techniques"?; What questions can biophysical techniques answer?; Which technique to use?; Organization of this book; 2 Molecular principles; 2.1 Molecules and interactions; Introduction; Box 2.1 Atoms and elements; Box 2.2 Kinetic model of gases; Non-covalent interactions; Box 2.3 Electrostatics, dielectrics, and polarity; Box 2.4 Molecular biology tools and base pairing; Binding; 3 Transport and heat; 3.1 Diffusion, osmosis, viscosity, and friction; Introduction; Diffusion; Osmosis

Application of a force to a molecule in solutionViscosity; Box 3.1 The frictional coeffi cient, f, depends on molecular shape; 3.2 Analytical centrifugation; Introduction; Some basic principles of sedimentation; Sedimentation velocity; Sedimentation equilibrium; Density gradient sedimentation; 3.3 Chromatography; Introduction; Theory; Chromatography techniques; Quantitative chromatography; 3.4 Electrophoresis; Introduction; Theory; Experimental; Some electrophoresis systems; 3.5 Mass spectrometry; Introduction; Ionization; Ion sorting/analysis; Applications of mass spectrometry

3.6 ElectrophysiologyIntroduction; Membrane potential; Action potentials; Experimental; Propagation of an action potential in a neuron; 3.7 Calorimetry; Introduction; Isothermal titration calorimetry; Differential scanning calorimetry; Box 3.2 Heat capacity; 4 Scattering, refraction, and diffraction; 4.1 Scattering of radiation; Introduction; Scattering theory; Box 4.1 Weight average molecular weight; Turbidity; Solution scattering and molecular shape; Box 4.2 Radii of gyration, R[sub(G)], and hydration, R[sub(H)]; Dynamic light scattering; Box 4.3 Correlation functions

4.2 Refraction, evanescent waves, and plasmonsIntroduction; Box 4.4 Classical optics; Evanescent waves; Surface plasmon resonance; Box 4.5 The streptavidin/biotin complex; 4.3 Diffraction; Introduction; Principles of diff raction; Box 4.6 Single particle imaging with X-ray laser; Diffraction experiments; Interpretation of diffraction data; Other crystallography techniques; Achievements of crystallography; 5 Electronic and vibrational spectroscopy; 5.1 Introduction to absorption and emission spectra; Introduction; Energy states; Absorption

Box 5.1 Transition dipole moments and transition probabilityEmission; Box 5.2 The laser; 5.2 Infrared and Raman spectroscopy; Introduction; IR spectra and applications; Raman scattering; Applications of Raman spectroscopy; 5.3 Ultraviolet/visible spectroscopy; Introduction; Measurement of electronic spectra; Electronic energy levels and transitions; Absorption properties of some key chromophores; Applications of UV/visible spectra; Box 5.3 Isosbestic points; Properties associated with the direction of the transition dipole moment; Monitoring rapid reactions; 5.4 Optical activity; Introduction

The phenomenon

Notes:

Previously issued in print: 2012.

Includes bibliographical references and index.

Description based on print version record.

ISBN:

0-19-197572-9

0-19-166614-9