Epigenomics, from chromatin biology to therapeutics / edited by Krishnarao Appasani ; forword by Azim Surani.

Format:

Book

Contributor:

Appasani, Krishnarao, 1959- editor.

Language:

English

Subjects (All):

Epigenetics.

Gene expression.

Physical Description:

1 online resource (xxx, 518 pages) : digital, PDF file(s).

Place of Publication:

Cambridge : Cambridge University Press, 2012.

Language Note:

English

Summary:

Understanding mechanisms of gene regulation that are independent of the DNA sequence itself - epigenetics - has the potential to overthrow long-held views on central topics in biology, such as the biology of disease or the evolution of species. High throughput technologies reveal epigenetic mechanisms at a genome-wide level, giving rise to epigenomics as a new discipline with a distinct set of research questions and methods. Leading experts from academia, the biotechnology and pharmaceutical industries explain the role of epigenomics in a wide range of contexts, covering basic chromatin biology, imprinting at a genome-wide level, and epigenomics in disease biology and epidemiology. Details on assays and sequencing technology serve as an up-to-date overview of the available technological tool kit. A reliable guide for newcomers to the field as well as experienced scientists, this is a unique resource for anyone interested in applying the power of twenty-first-century genomics to epigenetic studies.

Contents:

Cover; Epigenomics From Chromatin Biology to Therapeutics; Title; Copyright; Dedication; Contents; Contributors; Foreword; Preface; Part I Basics of chromatin biology and biochemistry; 1 Introduction to epigenomics; X-chromosome inactivation (dosage compensation); Genetic imprinting; Epigetic modifications and their mechanisms; Scope of this book; Part I: Basics of chromatin biology and biochemistry; Part II: Epigenomic imprinting and stem cells; Part III: Epigenomic assays and sequencing technology; Part IV: Epigenomics in disease biology; Part V: Epigenomics in neurodegenerative diseases

Part VI: Epigenetic variation, polymorphism, and epidemiological perspectivesCurrent issues in epigenomics; Identical twins; Future developments in epigenomics; References; 2 Epigenetics and its historical perspectives; 21 Introduction; 22 Inheritance in somatic cells; 23 Epimutations and dual inheritance; 24 Cancer and de novo methylation; 25 Chromatin remodeling; 26 Differences between genetic and epigenetic systems; 27 An epigenetic component in long-term memory?; 28 A wider definition of epigenetics; 29 The chronology of epigenetics; References

3 Functional networks of human epigenetic factors31 Introduction; 32 Methodology; 321 Design of reporter cell systems; 322 SiRNA-based knockdown analysis; 323 High-throughput siRNA screening; 324 Considerations for design and analysis of the siRNA screen; 33 Results and discussion; 331 Proof-of-concept study to identify factors and networks that participate in epigenetic gene silencing; 332 Design of siRNA library; 333 SiRNA high-throughput screening; 334 Identities of screen hits; 335 Evaluating position-specific versus epigenome-wide roles for silencing factors

336 Evidence for crosstalk between DNA methylation and histone modifications337 Interpreting output from epigenetic factor screens; 338 Identifying networks of "targetable" epigenetic factors; 34 Summary; Acknowledgements; References; 4 Nucleosome positioning in promoters: significance and open questions; 41 Introduction; 411 Micrococcal nuclease bias; 412 To sequence or not to sequence?; 42 Factors potentially affecting nucleosome positioning; 421 Pitfalls of sequence analysis; 422 Periodicity or artifact?; 423 DNA bendability and nucleosome positioning

424 Nucleosome positioning, histone modifications, and DNA methylation43 Hypotheses; 431 Transcription-coupled nucleosome positioning; 432 Chromatin structure as a cancer lineage classifier; 433 Retrotransposons and nucleosome positioning signals; 44 Concluding remarks; References; 5 Chemical reporters of protein methylation and acetylation; 51 Introduction; 52 Methodology; 521 Synthesis of chemical reporters; 522 In vitro transfer assays; 523 Labeling of cell lysates or living cells; 524 Determining the specificity of acetylation and methylation reporters

525 Detection of chemical reporters by click-chemistry

Notes:

Title from publisher's bibliographic system (viewed on 05 Oct 2015).

Includes bibliographical references and index.

ISBN:

1-107-22700-3

1-139-53976-0

1-283-52186-5

1-139-52695-2

9786613834317

1-139-52575-1

1-139-53161-1

0-511-77727-2

1-139-53042-9

1-139-52814-9

OCLC:

804664843