Transgenic animal technology : a laboratory handbook / edited by Carl A. Pinkert.

Format:

Book

Contributor:

Pinkert, Carl A.

Language:

English

Subjects (All):

Animal genetic engineering--Laboratory manuals.

Animal genetic engineering.

Physical Description:

1 online resource (635 p.)

Edition:

2nd ed.

Place of Publication:

Amsterdam ; Boston : Academic Press, c2002.

Language Note:

English

Summary:

DescriptionA transgenic animal is one that is genetically modified to carry genes from another species. Transgenic species can be raised to carry potentially useful genes from a variety of species. While the topics of genetic engineering and cloning are controversial, the reality is that these technologies offer tremendous benefits to society - from offering a framework for developing and screening medical therapies, to enhancing the safety and nutrition of the food we eat. Importance of TopicOne potential application of research into transgenic animal technology is the creatio

Contents:

Front Cover; Transgenic Animal Technology: A Laboratory Handbook; Copyright Page; Table of Contents; Contributors; Preface; PART 1: Overview; Chapter 1. Introduction to Transgenic Animal Technology; I. Introduction; II. Historical Background; III. Applications and Overview of Text; References; PART 2: Transgenic Animal Production Focusing on the Mouse Model; Chapter 2. DNA Microinjection and Transgenic Animal Production; I. Introduction; II. General Methods; III. Summary; Acknowledgments; References; Appendix; Chapter 3. Factors Affecting Transgenic Animal Production; I. Introduction

II. Getting StartedIII. Troubleshooting; IV. Transgenic Phenomenology; V. Summary; Acknowledgments; References; Chapter 4. Gene Targeting in Embryonic Stem Cells:; I. Stem Cells of the Embryo; II. Gene Targeting in Embryonic Stem Cells; III. Methods; Acknowledgments; References; Chapter 5. Gene Targeting in Embryonic Stem Cells:; I. Introduction; II. Conditional Technologies; III. Cre-loxP System Review, Development and Testing; IV. Cre-loxP and Transgenic Models; V. Methods; VI. Summary; Acknowledgments; References; Chapter 6. Retrovirus-Mediated Gene Transfer Teoan Kim

I. Introduction and DiscussionII. Methods; Acknowledgment; References; Chapter 7. Nuclear Transfer Technologies Yukio Tsunoda and Yoko Kato; I. Introduction and Discussion; II. Materials and Equipment; III. Preparation of Microtools; IV. Nuclear Transfer of Preimplantation Embryos; V. Nuclear Transfer of Embryonic Stem Cells and Somatic Cells; Acknowledgment; References; PART 3: Production of Transgenic Laboratory and Domestic Animal Species; Chapter 8. Production of Transgenic Rats; I. Introduction; II. Importance of Rats; III. Contributions from Transgenic Rats; IV. Production

V. ConclusionsReferences; Chapter 9. Production of Transgenic Rabbits; I. Applications of Transgenic Rabbits; II. Manipulation of Rabbit Embryos for Production of Transgenics; III. Materials and Methods; References; Chapter 10. Production of Transgenic Fish; I. Introduction; II. Discussion; III. Methods of Gene Transfer; IV. Establishing and Maintaining Transgenic Lineages; V. Care and Containment of Transgenic Fish; VI. Future of the Transgenic Fish; Acknowledgments; References; Chapter 11. Production of Transgenic Poultry; I. Introduction and Discussion; II. Methods; III. Summary

ReferencesChapter 12. Production of Transgenic Swine by DNA Microinjection; I. Introduction and Discussion; II. Methods; III. Alternative Methods for the Production of Transgenic Swine; IV. Summary; References; Chapter 13. Production of Transgenic Ruminants by DNA Microinjection; I. Introduction; II. Production of Zygotes Suitable for Microinjection; III. Microinjection of Ruminant Zygotes; IV. Transfer of Microinjected Embryos; V. Areas of Application and Perspectives; Acknowledgments; References; Chapter 14. Production of Transgenic Nonhuman Primates; I. Introduction; II. Methods

III. Summary

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

0-08-057480-7

OCLC:

833766276