Mixed polymeric micelles for osteosarcoma therapy : development and characterization / Catarina Melim, Ana Figueiras, Ivana Jarak & Ana Isabel Santos.

Format:

Book

Author/Creator:

Melim, Catarina, author.

Figueiras, Ana, author.

Jarak, Ivana, author.

Santos, Ana Isabel, author.

Language:

English

Subjects (All):

Osteosarcoma--Treatment.

Osteosarcoma.

Micelles.

Physical Description:

1 online resource (92 pages)

Edition:

1st ed.

Place of Publication:

Singapore : Bentham Science Publishers, [2022]

Summary:

Osteosarcoma is a rare bone tumor that has a high incidence amongchildren and young adults. Despite recent therapeutic developments, osteosarcomastill presents major hurdles to achieving successful results, mainly due to thepresence of multi-drug-resistant cells.This monograph primarily aims to provide information about the basicscience behind the treatment of osteosarcoma along with experimental resultsfor a novel formulation that overcomes multidrug resistance, and therefore, mayserve as a viable treatment option. The book starts with an updated and conciseguide to the pathophysiology of the disease, while also introducing the readerto new therapies and materials (specifically chitosan, polyethyleneimine, poloxamers,poloxamines, and Pluronics®) used in the treatment process along with the aimsof the experiments present subsequently. Next, the book documents the materialsand methods used in developing polymeric micelles for delivering drugs toosteosarcoma sites. By explaining the basics of nanomedicine as a startingpoint, readers will understand how polymeric micelles act as facilitators ofdrug transport to cancer cells, and how one can synthesize a small stablemicelle (by creating derivatives of base nanomaterials), capable of activelytargeting osteosarcoma cells and overcoming multi-drug resistance. The chapterexplains the synthesis and characterization techniques of the materials used todevelop polymeric micelles.The results, a reflection of the conjugation of different experimentalsolutions initiated here, point to a modern route towards the search for a therapeutic solution for osteosarcoma.The simple, structured presentation coupled with relevant informationon the subject of micelle-based nanotherapeutic drug delivery make thismonograph an essential handbook for pharmaceutical scientists involved in thefield of nanomedicine, drug delivery,

cancer therapy and any researchersassisting specialists in clinical oncology for the treatment of osteosarcoma.

Contents:

Cover

Title

Copyright

End User License Agreement

Contents

Foreword

Preface

DISCLAIMER

FUNDING

CONSENT FOR PUBLICATION

CONFLICT OF INTEREST

ACKNOWLEDGEMENT

Abstract

Introduction

1. OSTEOSARCOMA

1.1. Pathophysiology

1.1.1. Genetic Alterations

1.2. Diagnosis and Treatment

1.2.1. Targeted Therapy

1.2. Challenges to Osteosarcoma Treatment

1.2.1. Multidrug Resistance

1.2.2. Active Targeting

2. RNA INTERFERENCE TECHNOLOGY

2.1. miRNAs

2.1. miRNA-145

3. GENE THERAPY

3.1. Viral and Non-viral Vectors 3.1.1. Polymeric Micelles

3.1.2. Cationic Polymers

3.1.3. Poloxamers and Poloxamines

3.1.4. Micelleplexes

3.1.5. Physicochemical Characterization

AIMS

Materials and Methods

1. MATERIALS

2. CHITOSAN COMPLEXED WITH PLURONIC® F68

3. CHITOSAN COMPLEXED WITH PLURONICS® F68 AND P123

4. SYNTHESIS OF PLURONIC® F68 DIACRYLATE

5. SYNTHESIS OF PLURONIC® F68 DIACRYLATE CONJUGATED WITH PEI (F68-PEI)

6. CHARACTERIZATION OF THE PREPARED POLYMERIC MICELLES

6.1. Evaluation of Particle Size and Zeta Potential

6.2. 1H-NMR Spectroscopy 6.3. Fourier-Transform Infrared Spectroscopy

6.4. Transmission Electron Microscopy

6.5. Statistical Analysis

Results and Discussion

1. CHITOSAN COMPLEXED WITH PLURONIC® F68

2. CHITOSAN COMPLEXED WITH PLURONICS® F68 AND P123

3. SYNTHESIS OF PLURONIC® F68 DIACRYLATE

4. SYNTHESIS OF PLURONIC® F68 DIACRYLATE CONJUGATED WITH PEI

5. PREPATION OF MIXED PLURONIC® F68-PEI AND PLURONIC® P123 MICELLES

6. MORPHOLOGICAL CHARACTERIZATION

Conclusion

FUTURE PERSPECTIVES

Annex

1. CHITOSAN COMPLEXED WITH PLURONIC® F68 2. SYNTHESIS OF PLURONIC® F68 DIACRYLATE CONJUGATED WITH PEI

ABBREVIATIONS

LIST OF ABBREVIATIONS

REFERENCES

Subject Index

Back Cover.

Notes:

Includes bibliographical references.

Description based on publisher supplied metadata and other sources.

Description based on print record.

ISBN:

9789815040371

9815040375

OCLC:

1314631433