Metagenomics : methods, applications and perspectives / Camilla Benedetti, editor.

Format:

Book

Contributor:

Benedetti, Camilla, editor.

Series:

Microbiology research advances series.

Microbiology Research Advances

Language:

English

Subjects (All):

Environmental geochemistry.

Physical Description:

1 online resource (205 p.)

Place of Publication:

New York : Nova Publishers, 2014.

Language Note:

English

Summary:

Metagenomic analysis has extraordinary potential to improve our understanding of microbial populations in their natural environment and identify novel genes of interest. The key feature of such analyses is that they are performed using metagenomic libraries constructed from total DNA isolated from a particular niche rather than a laboratory culture. Thus, metagenomic analyses potentially allow access to all the genetic resources present in an environment, regardless of whether or not they belong to microorganisms that can be cultured in the laboratory. Sequence-based metagenomic analyses rely

Contents:

""METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES""; ""METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES""; ""Library of Congress Cataloging-in-Publication Data""; ""CONTENTS""; ""PREFACE""; ""Chapter 1: POTENTIAL AND LIMITATIONS OF METAGENOMIC FUNCTIONAL ANALYSES""; ""ABSTRACT""; ""1. MICROBIAL DIVERSITY""; ""2. OVERVIEW OF METAGENOMIC LIBRARY CONSTRUCTION""; ""3. METAGENOMIC LIBRARY ANALYSES""; ""4. FUNCTIONAL SCREENS""; ""5. METAGENOMIC GENE EXPRESION""; ""6. FUTURE PROSPECTS FOR IDENTIFICATION OF METAGENOMIC FUNCTIONS""; ""REFERENCES""

""Chapter 2: METAGENOMICS: LIBRARY CONSTRUCTION AND SCREENING METHODS""""ABSTRACT""; ""1. INTRODUCTION""; ""2. EXTRACTION OF NUCLEIC ACIDS FROM ENVIRONMENTAL SAMPLES""; ""3. ENRICHMENT OF ENVIRONMENTAL SAMPLES""; ""4. CONSTRUCTION OF A METAGENOMIC LIBRARY""; ""5. SCREENING OF METAGENOMIC DATA""; ""CONCLUSION""; ""REFERENCES""; ""Chapter 3: THE USE OF ION TORRENT PGM FOR BACTERIAL DIVERSITY ANALYSES: THE STUDY CASE OF FIVE BRAZILIAN HYDROELECTRIC RESERVOIRS""; ""ABSTRACT""; ""INTRODUCTION""; ""CONCLUSION""; ""REFERENCES""

""Chapter 4: METAGENOMIC PROFILING FOR ASSESSING ENVIRONMENTAL HEALTHY""""ABSTRACT""; ""1. INTRODUCTION""; ""2. EVALUATING ANTHROPOGENIC IMPACTS THROUGH METAGENOMICS""; ""3. APPLICATIONS OF METAGENOMICS TO PUBLIC HEALTH""; ""REFERENCES""; ""Chapter 5: MINING NOVEL GENES AND ENZYMES OF UNCULTURED EUKARYOTIC MICROORGANISMS BY AN RNA-BASED APPROACH""; ""ABSTRACT""; ""METAGENOMICS: ACCESS TO THE UNCULTURED MICROBIAL RESOURCES""; ""TARGET OF METAGENOMIC ANALYSIS""; ""APPROACHES TO METAGENOMIC SCREENING""; ""EUKARYOTIC CELLS AS A TARGET RESOURCE FOR METAGENOMIC ANALYSIS""

""EXPLORING THE FUNCTIONAL EUKARYOTIC GENES BY A METATRANSCRIPTOMIC APPROACH""""CONCLUDING REMARKS AND FUTURE PROSPECTS""; ""REFERENCES""; ""Chapter 6: DEVELOPMENTS IN THE RETRIEVAL OF NOVEL BIOCATALYSTS BY METAGENOMIC APPROACHES""; ""ABSTRACT""; ""INTRODUCTION""; ""OVERCOMING THE PROBLEMS IN THE EXTRACTION AND OBTAINING HUMUS-FREE PURE ENVIRONMENTAL DNA""; ""PROBLEMS IN SCREENING METAGENOMIC LIBRARIES""; ""PROGRESS ACHIEVED IN DISCOVERING A VARIETY OF BIOCATALYSTS""; ""OTHER INDUSTRIALLY IMPORTANT ENZYMES""; ""SUCCESS STORIES AND COMMERCIALIZED METAGENOMIC BIOCATALYSTS""; ""CONCLUSION""

""REFERENCES""""Chapter 7: ISOLATION OF SOIL METAGENOMIC DNA: CHALLENGES AND SOLUTIONS""; ""ABSTRACT""; ""INTRODUCTION""; ""1. ORIGIN OF METAGENOMICS""; ""2. SOIL - THE ECOSYSTEM OF CHOICE FOR METAGENOMIC STUDIES""; ""3. ISOLATION OF METAGENOMIC DNA""; ""CONCLUSION""; ""REFERENCES""; ""Chapter 8: MICROBIAL EXPLORATION IN EXTREMECONDITIONS: METAGENOMIC ANALYSISAND FUTURE PERSPECTIVES""; ""ABSTRACT""; ""INTRODUCTION""; ""LIMITATIONS OF CONVENTIONAL MICROBIAL METHODS""; ""SANGER AND NEXT GENERATION SEQUENCING""; ""THE METAGENOMICS PIPELINE""; ""BIOINFORMATICS AND THERMOPHILE METAGENOMICS""

""ANNOTATION OF METAGENOMIC SEQUENCES""

Notes:

Description based upon print version of record.

Includes bibliographical references at the end of each chapters and index.

Description based on print version record.

ISBN:

1-63321-698-5

OCLC:

914433442