Endocrine methods / edited by John A. Thomas.

Format:

Book

Contributor:

Thomas, J. A. (John A.), 1933-

Language:

English

Subjects (All):

Endocrinology--Methodology.

Endocrinology.

Endocrine toxicology--Methodology.

Endocrine toxicology.

Hormones--Analysis.

Hormones.

Physical Description:

1 online resource (483 p.)

Place of Publication:

San Diego : Academic Press, c1996.

Language Note:

English

Summary:

Endocrine Methods contains descriptions of contemporary and cutting-edge methodologies in various areas of endocrinology, including receptor theory and immunologic techniques for endocrine research. The book presents step-by-step procedures easily available to study the endocrine system and includes experts in their respective fields as contributors. The book presents step-by-step procedures for many important areas of endocrine target organs.Endocrine Methods serves as a valuable methodological resource for investigators using endocrine methods.Includes comprehensive

Contents:

Front Cover; Endocrine Methods; Copyright Page; Contents; Contributors; Preface; Chapter 1. Assay Techniques Available for the Toxicologist; Introduction; Colorimetric Assays; Bioassays; Radioreceptor Assays; Radioimmunoassay; Nonradiometric Assays; Fluorescence Assays; Chemiluminescent Assays; Other Assay Techniques; Appendix 1: Selected Hormone Marker Assays; Conclusion/Addendum; References; Chapter 2. Longitudinal Bone Growth: Experimental Methods; Principles of Longitudinal Bone Growth; Measurement of Longitudinal Bone Growth; Growth Plate Infusion; References

Chapter 3. Measurement of Steroid Receptor Binding to DNAIntroduction; Mobility Shift Analysis; DNase I Footprinting; Methylation Interference; References; Chapter 4. Hypophysical Grafts beneath the Renal Capsule: A Model to Study Endocrine Control Systems; History and Utility of Pituitary Grafts; Comparison of the Pituitary Allograft Model with Studies Using Pituitary Tissue in Situ or in Vitro; Techniques and Considerations for Grafting Pituitary Tissue beneath the Renal Capsule; Summary of the Status of the Host and the Allograft Tissue; References

Chapter 5. Prolactin Secretion from Single Cells: Analysis by Reverse Hemolytic Plaque AssaysIntroduction; Establishment of a Prolactin Plaque Assay; Procedures; Advantages of Using RHPA Analysis; Application of Plaque Assays to Assess Toxic Influence; References; Chapter 6. Application of Fluorescence Techniques to Bone Biology; Introduction; Cell Cultures; Analyses in Cell Populations; Analyses in Single Cells; References; Chapter 7. Measurement of Thyroid Hormone and Related Molecules; Introduction; Laboratory Measurements to Assess Thyroid Function

Autoimmune Processes That Influence Thyroid Gland Function and Hormone Measurements: Serum Thyroid AutoantibodiesMeasurement of Serum Thyroglobulin; Conclusions; References; Chapter 8. Uptake Measurements of Thyroid Hormones and Amino Acidds in Cultured Cells; Introduction; Amino Acid Transport Systems; Uptake Measurements in Cultured Cells; Plasma Membrane Vesicles Derived from Cultured Cells for Uptake Measurements; Conclusion; References; Chapter 9. Two-Dimensional Gel Electrophoresis of Proteins; Introduction; Sample Preparation; The First Dimension: The ISO System

Alternate First Dimension: The IPG SystemThe Second Dimension: The DALT System; Detection of Protein Spots in 2D Gels; Protein Blotting; Analysis of 2D Protein Profiles by Computer- Based Image Analysis Systems; Summary; References; Chapter 10. Effects of in Vivo Exposure to Glucocorticoids on Pituitary, Serum, and Messenger Ribonucleic Acid Levels of the Gonadotropin Hormones FSH and LH in Male and Female Rats; Introduction; Methodologies; Experimental Protocols; Results; Summary; References; Chapter 11. Glutamate Receptor Autoradiography and in Situ Hybridization; Introduction

Methods for Studying Glutamate Receptor Expression

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-281-04991-3

9786611049911

0-08-053089-3

OCLC:

476112130