Nonviral vectors for gene therapy / edited by Leaf Huang, Mien-chie Hung, Ernst Wagner.

Format:

Book

Contributor:

Huang, Leaf.

Hung, Mien-chie.

Wagner, Ernst, 1960-

Language:

English

Subjects (All):

Gene therapy.

Genetic vectors.

DNA-ligand interactions.

Liposomes.

Drug targeting.

Genetic transformation.

Physical Description:

1 online resource (471 pages)

Place of Publication:

San Diego, CA : Academic Press, c1999.

Language Note:

English

Summary:

Gene transfer within humans has been an obstacle until about 10 years ago. At that time, it was found that viral vectors were effective carriers of ""healthy genes"" into patients' cells. The problem, however, was that viral vectors proved unnecessarily harmful to humans: subjects experienced inflammatory activity and negative immunological responses to the genes. Viral vectors were also unable to meet the needs of the pharmaceutical community: they were not reproducible in large-scale proportions in cost-effective ways.

Contents:

Front Cover; NONVIRAL VECTORS for GENE THERAPY; Contents; Foreword by Jean-Marie Lehn; Foreword by John Mendelsohn; Contributors; PART I: INTRODUCTION; Chapter 1. Introduction; I. Gene Therapy; II. Virus vs Nonvirus as a Vector; III. Characteristics of a Nonviral Vector; IV. Delivery Barriers That a Vector Must Overcome; V. Cytoplasmic Expression; VI. Hybrid Viral/Nonviral Vectors; VII. Conclusion; References; PART II: CATIONIC LIPOSOMES; Chapter 2. Progress in Gene Delivery Research and Development; I. Early in Vitro Transfection Studies; II. Molecular Biology and Biotechnology Applications

III. Application of Synthetic Vectors for Gene Therapy; IV. Developing More Efficient Synthetic Gene Delivery Systems; References; Chapter 3. Cationic Lipid-Mediated Gene Delivery to the Airways; I. Introduction; II. The Lung as a Target for Gene Transfection; III. Cystic Fibrosis; IV. Cationic Lipids for CF Gene Therapy; V. Efficiency of Cationic Lipids at Mediating Gene Transduction to the Lung; VI. Limitations of Present Cationic Lipid Formulations; VII. Clinical Studies; VIII. Summary; References; Chapter 4. Structure and Structure-Activity Relationships of Lipid-Based Gene Delivery Systems

I. Introduction and History; II. Structure-Activity Relationships; III. Conclusion and Future Prospects; References; Chapter 5. Self-Assembled Structures of Lipid/DNA Nonviral Gene Delivery Systems from Synchrotron X-Ray Diffraction; I. Introduction; II. Synchrotron X-Ray Diffraction Studies; III. Future Directions; Acknowledgments; References; Endnote; Chapter 6. Sites of Uptake and Expression of Cationic Liposome/DNA Complexes Injected Intravenously; I. Introduction; II. Methodology; III. Interactions of Liposomes and Liposome/DNA Complexes with Blood; IV. Liposome Uptake in the Vasculature

V. Liposome Uptake in Angiogenic Blood Vessels; VI. Tissue-Specific Expression of Reporter Genes; VII. Implications for the Use of Cationic Liposomes for DNA Delivery; Acknowledgments; References; PART III: OTHER VECTORS; Chapter 7. Nuclear Transport of Exogenous DNA; I. Introduction; II. The Nuclear Transport of Macromolecules; III. The Nuclear Transport of Exogenous DNA; IV. Attempts to Increase the Efficiency of Nuclear Uptake; V. Viral Strategies for DNA or RNA Nuclear Transport; VI. Conclusions and Future Prospects; References

Chapter 8. Particle-Mediated Gene Delivery: Applications to Canine and Other Larger Animal Systems; I. Introduction; II. Ex Vivo Gene Transfer into Canine Tumor Cell Explants and Derived Primary Cultures; III. Ex Vivo Gene Transfer to Normal Canines Using Established Melanoma Cell Lines; IV. Clinical Trial of ex Vivo Transfected Autologous Cancer Vaccines in Spontaneous Tumors in Canines; V. In Vivo Gene Transfer into Skin and Oral Mucosal Epithelial Cells in Normal Dogs; VI. Gene Gun Technology as a Delivery Mechanism DNA Vaccines; VII. Conclusions; References; Chapter 9. Polyethylenimines. A Family of Potent Polymers for Nucleic Acid Delivery

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-281-05043-1

9786611050436

0-08-047977-4

OCLC:

476057770