GFP whole cell microbial biosensors : scale-up and scale-down effects on biopharmaceutical processes / Frank Delvigne ... [et al.].

Format:

Book

Contributor:

Delvigne, Frank.

Series:

Biomedical and nanomedical technologies.

Biomedical & nanomedical technologies

Language:

English

Subjects (All):

Microbial biotechnology.

Green fluorescent protein.

Biosensors.

Physical Description:

1 online resource (54 p.)

Place of Publication:

New York, N.Y. : ASME ; New York, N.Y. (222 East 46th Street, New York, NY 10017) : Momentum Press, 2013.

Language Note:

English

Summary:

Two strategies are usually considered for the optimization of microbial bioprocesses. The first one involves genetic or metabolic engineering of the target microbial strains in order to improve its production efficiency or its tolerance to adverse conditions. The second one is based on the chemical engineering improvement of the bioreactors and scaling-up rules. This work is more particularly dedicated to this second class of parameters.

Contents:

1. Interaction between fluid flow and microbial cells: importance of the operating scale

1.1 Different scales involved in the development of microbial bioprocesses

1.2 Hydrodynamic modeling of the substrate heterogeneites perceived by cells

2. Stochastic simulation of the displacement of microbial cells along concentration field

2.1 Microbial stress response: different timescales involved

3. Experimental results gained from the physiological response of GFP biosensors in scale-down conditions

3.1 General stress response in scale-down bioreactors: rpoS::GFP

3.2 Use of destabilized GFP variants

4. Another source of information: protein leakage and the study of the secretome

References.

Notes:

Description based upon print version of record.

Includes bibliographical references (p. 36-43).

Title from PDF t.p. (viewed on January 27, 2013).

ISBN:

1-283-94834-6

1-60650-417-7

OCLC:

827211691