Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing / edited by Ana M. Aransay, José Luis Lavín Trueba.

Format:

Book

Contributor:

Aransay, Ana M., Editor.

Lavín Trueba, José Luis., Editor.

Language:

English

Subjects (All):

Human genetics.

Systems biology.

Biometry.

Human Genetics.

Systems Biology.

Biostatistics.

Local Subjects:

Human Genetics.

Systems Biology.

Biostatistics.

Physical Description:

1 online resource (404 p.)

Edition:

1st ed. 2016.

Place of Publication:

Cham : Springer International Publishing : Imprint: Springer, 2016.

Summary:

High throughput sequencing (HTS) technologies have conquered the genomics and epigenomics worlds. The applications of HTS methods are wide, and can be used to sequence everything from whole or partial genomes, transcriptomes, non-coding RNAs, ribosome profiling, to single-cell sequencing. Having such diversity of alternatives, there is a demand for information by research scientists without experience in HTS that need to choose the most suitable methodology or combination of platforms and to define their experimental designs to achieve their specific objectives. Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing aims to collect in a single volume all aspects that should be taken into account when HTS technologies are being incorporated into a research project and the reasons behind them. Moreover, examples of several successful strategies will be analyzed to make the point of the crucial features. This book will be of use to all scientist that are unfamiliar with HTS and want to incorporate such technologies to their research.

Contents:

The High Throughput Sequencing technologies triple-W discussion: Why to use HTS, What is the optimal HTS method to use, Which data analysis workflow to follow

Whole genome sequencing recommendations

Counseling for targeted DNA region re-sequencing

Transcriptome profiling strategies

Differential mRNA Alternative Splicing

microRNA discovery and expression analysis in animals

Analysis of Long Non-coding RNAs in RNA-seq data

Ribosome profiling

Genome-wide analysis of DNA methylation patterns by high-throughput sequencing

Characterization of DNA-protein interactions: Design and analysis of ChIP-seq experiments

PAR-CLIP: A genomic technique to dissect RNA-protein interactions

Metagenomic design and sequencing

A hitchhiker's guide to Metatranscriptomics

Eukaryotic Single-Cell mRNA Sequencing

Eukaryotic Single-Cell DNA Sequencing

Submitting data to a public repository, the final step of a successful HTS experiment.

Notes:

Description based upon print version of record.

Includes bibliographical references at the end of each chapters and index.

ISBN:

3-319-31350-9